萝卜硫素（SF）是目前发现的抗癌活性最强的植物源天然次生代谢物，西兰花是SF提取率最高的植物材料，但对其合成的信号调控机制缺乏系统研究。该研究利用细胞工程和基因工程技术获得的西兰花毛状根为受体材料，运用信号分子水杨酸、茉莉酸和茉莉酸甲酯处理，测定萝卜硫苷（GRA）含量，确定诱导GRA合成的最佳信号分子及其浓度，检测最佳信号分子诱导下GRA合成通路中关键基因与酶蛋白表达量；调节芥子酶（MYR）、表皮特异硫蛋白（ESP）活性，检测毛状根中GRA和SF含量的变化，阐明最佳信号分子诱导西兰花毛状根GRA合成代谢的调控网络，确定西兰花毛状根GRA向SF转化量最大时MYR、ESP活性调控的最佳处理，为利用西兰花毛状根进行规模化培养生产SF提供理论基础和技术保障。

Sulforaphane（SF）was discovered as plant natural secondary metabolite which had best anti-cancer activity, Broccoli was the plant material with the highest extraction rate of SF, but the signal control mechanism of SF synthesis was lack of systematic research. This experiment used Broccoli hairy roots which was obtained by the technology of cell and genetic engineering as acceptor material, measured the content of Glucoraphanin（GRA）, determined the optimum signal molecule and concentrations which induced GRA synthesis, researched key genes and enzyme protein expressions in GRA Synthetic pathways induced by optimum signal molecule; measured the content changes of GRA and SF through regulating the activity of myrosase（MYR）and Epidermal specific thioredin protein(ESP), illustrated the regulatory networks of GRA anabolism induced by optimum signal molecule in Broccoli hairy roots, confirmed the optimum activity regulation of MYR and ESP when GRA had maximum resultant quantity to SF, provided theoretical basis and technical support for manufacturing SF by large-scale cultivation Broccoli hairy roots.