水稻转基因技术为水稻育种提供了一个崭新的重要途径，同时也是水稻基因功能研究的重要手段。目前，已经建立了粳稻品种的高效遗传转化体系，大大加快了转基因技术在粳稻中的应用。然而，籼稻的愈伤组织诱导及植株再生频率都很低, 限制了转基因技术在籼稻中的应用。在前期研究中，我们通过蛋白质组学分离到一个与水稻愈伤组织分化及再生有关的蛋白ISR。在此基础上，本研究将围绕ISR基因的功能展开相关研究，利用遗传学、分子生物学、生物化学、转基因等手段研究该基因是如何参与调控水稻愈伤组织分化及再生。这些研究将为解决籼稻愈伤组织诱导和植株再生率低提供理论依据，同时也为我国转基因技术在籼稻育种中的应用打下理论基础。

The rice genetic transformation is a powerful tool for rice improvemnent and functional genomics study. Establishment of a highly efficient tissue-culture system has greatly accelerated the wide spread application of transgenic japonica varieties. However, the callus induction and plant regeneration frequency are low in indica, limiting the application of genetic engineering in indica varieties. In preliminary studies, we isolated a protein which is related with the rice callus differentiation and regeneration. Here, we will study the function of ISR genes by genetics, molecular biology, biochemistry, transgenic and other methods. This work will provide us more understanding about the mechanism of rice callus differentiation and regeneration, and it will be helpful to the research of the transgenic in indica varieties.