调控寄生蜂滞育行为是实现寄生性天敌产品的长期贮存、延长控害时间和提高抗逆性的重要手段。但以平腹小蜂为代表的寄生蜂滞育机制研究缺乏，严重制约其产业化。申请人前期研究发现低温短光照可导致日本平腹小蜂进入滞育状态，首次明确了该虫属于幼虫滞育类型，但是平腹小蜂滞育调控的分子机制尚不明确。本研究拟利用前期获得的滞育、非滞育和滞育解除平腹小蜂为研究材料，通过三重四级杆质谱（UHPLC-QQQ-MS）和qRT-PCR技术，明确滞育不同阶段保幼激素滴度变化及保幼激素应答基因的表达差异，然后通过RNAi技术干扰滞育平腹小蜂的靶标基因，检测干扰后保幼激素的滴度变化及平腹小蜂表型变化，明确靶标基因在调控保幼激素滴度和平腹小蜂滞育中的作用，揭示保幼激素应答基因调控平腹小蜂幼虫滞育的分子机制。预期结果将为深入研究和解析平腹小蜂幼虫滞育的机理奠定基础，为精确调控天敌昆虫发育适应生产应用提供理论依据。

Diapause regulation of parasitic wasps is an important strategy to realize the long time storage of the species, prolong the time of prevention and control and improve the rersistance of the products. But there are few studies on molecular mechanism of diapause in parasitic wasps, which restricts their industrialization. Low temperature and short photoperiod can induce A. japonicas larvae into diapause at mature larvae state, but the molecular mechanism of diapause is still unclear. In this study, we will detect the changes of juvenile hormone titer and the expression profile of juvenile hormone response genes, at different stages of diapause in A. japonicas, using UHPLC-QQQ-MS and qRT-PCR. RNAi technology was used to interfere with the target genes, to clarify the function of the target genes in regulating juvenile hormone titer and the diapause in A. japonicas. The results will reveal the molecular mechanism of juvenile hormone response genes in regulating the larval diapause of A. japonicas and provide theoretical support for accurately regulating the development of natural enemy and meet the needs of application.