癌症的早期诊断是化学和生物医学等交叉学科的一个重要研究方向,作为检测癌症的手段—免疫传感器也越来越受到科研工作者的广泛关注。针对目前无标记免疫传感器表面活性位点少导致肿瘤标志物的固定化困难和蛋白质在免疫传感器表面的非特异性吸附造成的背景干扰大、灵敏度降低和假阳性信号出现这两大问题，本项目在前期仿生聚合物研究工作的基础上，利用聚多巴胺的粘附性、多官能团性和还原性，制备了聚多巴胺-Ag纳米粒子-聚多巴胺复合膜，并将具有抗生物污垢功能的聚两性离子-丙烯酸共价键合到聚多巴胺-Ag纳米粒子-聚多巴胺复合膜表面，用于固定肿瘤标志物，制备一种新型的具有高灵敏度和抗生物污垢能力的无标记免疫传感器。有望为解决无标记免疫传感器实现复杂样品的高灵敏检测提供理论指导和实验依据,具有重要的科学意义和应用价值。

Early diagnosis of cancer is an important interdisciplinary filed of chemistry and biomedical. As detection mean of cancer, immunosensor has aroused widespread concern of researchers. Less active site on the surface of label-free immunosensor, made tumor markers immobilized difficulty. Even more, in the detection of complex biological samples, due to nonspecific protein adsorption on label-free immunosensor resulting in high level background interferencefalse，false positive signal and sensitivity decreased. Aiming at those problems, based on our previous work of biomimetic polymer research, the polydopamine with the property of adhesion，polyfunctional and reducing was used to form a polydopamine-Ag nanoparticles-polydopamine composite film. For the tumor markers immobilization, anti-biofouling zwitterionic copolymers were introduced onto the surface of polydopamine-Ag nanoparticles-polydopamine composite film, which endows high sensitivity and anti-biofouling ability of label-free immunosensor. The preparation of new label-free immunosensor has a good theoretical guidance and practical significance in complex samples high sensitivity detection, which has important scientific significance and application value.