鼻粘膜上皮结构及功能紊乱(组织重塑)是慢性鼻窦炎(chronic rhinosinusitis,CRS)发病的核心病理特征之一，上皮间质样转化(epithelial-to-mesenchymal transition, EMT)是上皮组织重塑的关键环节。申请人前期在国自然项目的资助下，开展全基因组关联研究发现位于碱性核蛋白1(basonuclin1, BNC1)基因的多态性与CRS发生相关，BNC1在CRS鼻窦粘膜上皮全层表达，且较正常粘膜表达增高，BNC1可能通过影响鼻窦粘膜上皮功能在CRS发生中发挥作用。本课题拟从组织-细胞-动物三个层次深入研究，探讨CRS上皮EMT程度与BNC1表达变化的内在联系，并揭示BNC1在转化生长因子beta1/smad3通路介导的鼻粘膜上皮EMT过程的具体分子机制。本研究将有助于深入阐明CRS上皮重塑的分子机制，为CRS的防治提供新的分子靶标和新思路。

Nasal epithelial structure and function disorders (tissue remodeling) is one of the core pathological features of the development of chronic rhinosinusitis (CRS), while epithelial-to-mesenchymal transition (EMT)is the key point of tissue remodeling. The applicant carried out pooling based Genome wide association study and found a significantly association between basonuclin1 (BNC1) gene polymorphisms and CRS with funding from National Science Fund. Moreover, is was demonstrated that BNC1 expressed in the whole layer of sinus mucosa epithelium in CRS subjects and exhibited a significantly higher mRNA level compared with healthy control nasal mucosa. In addition, it has been proofed that BNC1 may play a role in the occurrence of CRS through influencing sinus mucosa function. The aim of present study is to investigate the interior relation between BNC1 expression changes and EMT degree of the sinus epithelium in patients suffering from CRS through three aspects which include tissue, cell and animal to reveal the potential mechanisms of BNC1 in the nasal epithelial EMT process mediated by transforming growth factor beta1 / smad3 pathway. The research will help to elucidate the molecular mechanisms of epithelial remodeling of CRS and provide new molecular targets and new ideas regarding the prevention and treatment of CRS.