瑶族医药以疗效取胜，但系统性深入研究较少。桃金娘科常用瑶药用于多种炎症的治疗，其效果得到长期临床验证和认可，并且均含有黄酮类化合物，是主要抗炎活性成分。对岗松前期研究表明，C-甲基化黄酮是桃金娘科植物特征性的化学成分，具有良好的生物活性；由岗松中C-甲基化黄酮化学结构新颖多样性，以及类似酚酸偶合反应的生源途径推测，桃金娘科瑶药中依然存在结构新颖的活性C-甲基化黄酮类衍生物。本课题拟在前期工作基础上，基于HPLC-DAD-Q-TOF/MS技术，快速定向分离鉴定桃金娘科瑶药中新颖结构C-甲基化黄酮类成分；并结合药物功效及临床应用特点，采用现代药理模型与方法，从细胞/分子/动物水平探讨其抗炎作用及机制。该研究成果在丰富C-甲基化黄酮天然产物化学结构多样性，发掘具有潜在药用价值全新化合物的同时，阐明桃金娘科瑶药抗炎作用物质基础，这对我国民族医药资源开发和具有自主知识产权新药研发具有重要意义。

As an important part of Traditional Chinese Medicine, Yao medicines are famous for their mysterious curative effects on difficult miscellaneous diseases, but are lack of systematic and in-depth studies. Myrtaceae Yao medicines are used for the treatments of various inflammatory and their efficacies are validated by long-term clinical applications. To our knowledge, flavonoids are the main bioactive constituents of Myrtaceae medicinal plants. Our previous investigations of Baeckea frutescens L. revealed that C-methylated flavonoids might be distinctive of the family Myrtaceae and exhibited a variety of biological functions. The diversities of novel chemical structures and biosynthetic pathways (phenolic coupling reaction) of isolated C-methylated flavonoids suggested that novel and bioactive C-methylated flavonoids derivatives must exist in all species of Myrtaceae Yao medicines. This project is proposed on the basis of our preliminary work and focused on novel C-methylated flavonoids and their anti-inflammatory activity. From the points of time consuming and accurate mass measurement capability, HPLC-DAD-Q-TOF/MS is employed for screening and guiding target separation of new compounds in herbal extract. Considering traditional efficacy and clinical characteristics, modern pharmacological models and methods are used to investigate anti-inflammatory effects and mechanisms at cells, molecules and animal levels respectively. The results of current project will discover novel C-methylated flavonoids with potential medicinal value and elucidate the anti-inflammatory therapeutic material basis for Myrtaceae Yao medicines. Furthermore it is of great significance for esearch and development of new drugs with independent intellectual property rights and resource development of traditional minority medicine.