肿瘤细胞对酸性微环境的适应与其增殖、迁移和侵袭等行为密切相关，目前，许多质子感受器已然成为新型肿瘤治疗药物的潜在靶点。本项目前期工作中发现：胞外酸性诱导野生型小鼠胚胎成纤维细胞（MEF）内Ca2+浓度升高，一种未知的质子感知Gq蛋白偶联受体（GqPCR）参与此调控过程。但这种未知的质子感知GqPCR是什么？目前尚不清楚。本项目拟通过正向遗传学筛选策略（自发突变和人工诱变）、全基因组测序、mRNA测序、Taqman GPCR array、RNAi、细胞转染和定点突变等实验技术和手段，对前期工作中发现的新的质子感知GPCR进行筛选并对其质子感知功能进行鉴定。本项目的开展，可能揭示具有质子感知功能的新组分，为细胞膜上更多质子感受器的发现提供新的思路，为新型抗癌药物的开发提供新的靶点。

The adaptation of tumor cells to acidic microenvironment is closely related to their proliferation, migration and invasion. At present, many proton sensors have become potential targets for tumor therapy. Our previous study found that in wild-type mouse embryonic fibroblasts (MEF), there is an unknown proton-sensing Gq protein-coupled receptor (GqPCR), which senses extracellular acidification and increases the intracellular Ca2+ concentration. But it is currently still elusive what this noval proton-sensing GqPCR is. This project is intended to screen and identify this noval proton-sensing Gq protein-coupled receptor found in MEF, using methods of forward genetics strategy (natural mutation and artificial mutagenesis), whole genome sequencing, mRNA sequencing, Taqman GPCR array, RNA interference, cell transfection and site-directed mutagenesis. This study may reveal new components with proton-sensing function, providing new ideas for the discovery of more proton sensors on the cell membrane, and providing new targets for the development of new anticancer drugs.