促进伤口愈合是横跨医学众多领域的关键科学问题，已有研究表明电场能够促进伤口愈合，并且电场通过指导细胞定向迁移促进伤口修复。然而对于电场介导细胞定向迁移的具体分子机制了解还不是十分清楚。本项目拟以参与伤口修复过程的人皮肤角质形成细胞（HaCat）为研究对象，在体外环境下向细胞施加模拟活体环境下的微小直流电场，再通过转录组测序和蛋白质组关联分析的方法比较基因表达差异，筛选与细胞趋电迁移有关的基因选出作为候选基因，进而通过qRT-PCR、Western-Blot、目的基因干扰及过表达等技术验证，挖掘调控人皮肤角质形成细胞（HaCat）趋电迁移的关键基因及其上下游通路，分析关键分子机制。研究结果不仅为上皮细胞趋电迁移的分子机制的建立提供实验证据，而且为电刺激促进伤口修复提供理论依据。

How to promote wound healing is an important scientific question in medical domain. Scientists indicate electric field promote wound healing through guide cells’ directional migration. But it is still not very clear about the molecular mechanism of how electric fields guide cells’ directional migration. In this project, we plan to take human skin keratinocytes (HaCat) as research object, apply small direct current(DC) electric field which is mimic the environment in-vivo on cells, using RNA-sequence and proteome sequencing to compare the difference of cells’ gene expression in two treatment(with electric field and with no electric field), select genes which relate to cell's electrotaxis as candidate genes, and validate through qRT-PCR, Western Blot, target gene interference and over expression to investigate the key genes that regulate electric field guided cells’ directional migration, analyze the mechanism. Meanwhile, this project not only provides experiment evidence for molecular mechanism of epidermal cells' electrotaxis, but also provides theoretically support for electrical stimulation promotes wound healing.