南方水稻黑条矮缩病毒诱导的一种新型胞间通道结构与功能解析

批准号:
31972980
项目类别:
面上项目
资助金额:
58.0 万元
负责人:
张恒木
依托单位:
学科分类:
植物病理学
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
张恒木
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中文摘要
南方水稻黑条矮缩病毒(SRBSDV)是近年才发现的一个威胁水稻安全生产的病毒新种。前期我们在其病株韧皮部筛管细胞-筛管细胞(SE-SE)或筛管细胞-韧皮部薄壁细胞(SE-PP)界面上首次发现了一种新型的细胞间通道,暂命名为FL通道,其结构特性明显区别于已知的2类植物细胞间通道:胞间连丝(PD)和筛孔(SP)。为了深入理解这个新通道的结构与功能,本研究拟联合浙江大学一起以SRBSDV诱导的FL通道为模式研究对象,综合采用电子断层成像(ET)、原子力显微镜(AFM)、聚焦离子束-扫描电镜(FIB-SEM)、自动化带式切片机扫描电镜(ATUM-SEM)和分子生物学技术进一步解析FL通道的形态结构、力学性能、分布模式及其在SRBSDV胞间运动中的功能特性,从而拓展细胞间通道和大尺寸病毒在植物体内运动的知识,为发展控制病毒病的新策略奠定基础。
英文摘要
Southern rice black-streaked dwarf virus (SRBSDV), a novel species of virus found on rice plants, has been threatening the production of rice in recent years. In our previous study, a new type of intercellular gateways, tentatively named flexible (FL) gateways, were first found on the sieve element-sieve element (SE-SE) or sieve element-phloem parenchyma (SE-PP) interfaces of phloem in infected rice plants. Its structural properties were obviously different from plasmodesma (PD) and sieve pore (SP), two types of known Intercellular gateways in plants. To gain a deep insight into its structure and function, the FL gateway induced by SRBSDV will be used as a model in this study and its morphology and structures, mechanical property, distribution pattern, and functional properties in cellular movement of SRBSDV will be further investigated by the combination of molecular biotechnology with a series of microscopic imaging, including electron tomography (ET), atom force microscopy (AFM), focus ion beam-scan electron microscopy (FIB-SEM), and automatic tape-collecting ultramicrotome-scan electron microscopy (ATUM-SEM). This study could be contributed to extend the knowledge of intercellular gateways and the movement of large-sized viruses within plants, and lay the foundation for developing the new strategy to control the viral diseases.
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专利列表
DOI:10.16819/j.1001-7216.2023.230108
发表时间:2023-11-01
期刊:Zhongguo Shuidao Kexue
影响因子:--
作者:Qi, Panpan;Guo, Liuming;Zhang, Hengmu
通讯作者:Zhang, Hengmu
DOI:10.1111/nph.19319
发表时间:2023
期刊:New Phytologist
影响因子:--
作者:Lv MF;Dai YX;Xie L;Guo JS;Liao ZF;Shang WN;Zhao XH;Hong J;Zhang HM
通讯作者:Zhang HM
DOI:10.1111/mpp.13277
发表时间:2023-01
期刊:MOLECULAR PLANT PATHOLOGY
影响因子:4.9
作者:Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Liao, Qiansheng;Xie, Li;Zhang, Heng-Mu
通讯作者:Zhang, Heng-Mu
A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms
水稻的 I 类胞质 HSP20 增强不同生物体的耐热性和耐盐性
DOI:10.1038/s41598-020-58395-8
发表时间:2020-01-28
期刊:SCIENTIFIC REPORTS
影响因子:4.6
作者:Guo, Liu-Ming;Li, Jing;Zhang, Heng-Mu
通讯作者:Zhang, Heng-Mu
南方水稻黑条矮缩病毒致瘤过程与分子机制研究
- 批准号:--
- 项目类别:联合基金项目
- 资助金额:258万元
- 批准年份:2020
- 负责人:张恒木
- 依托单位:
国内基金
海外基金
