微生物修复是去除土壤PAEs污染的重要手段。外源微生物的低环境适应性严重限制了实际应用，诱导强化土著微生物原位修复已成为当前研究热点。本项目以蚯蚓为工程生物，在探明“土壤动物和土著微生物”协同作用下土壤PAEs生物降解效果和路径基础上，利用DNA-SIP和高通量测序，研究蚯蚓强化的土壤PAEs降解过程中降解微生物种类，尤其是参与酯键水解的关键微生物。利用宏转录组，分析PAEs降解过程中表达量显著变化的基因，筛选蚯蚓强化下参与PAEs降解的关键（新）基因，结合经典分子生物学技术对其进行功能验证。利用宏基因组技术，探明关键（新）基因来源于何种微生物，结合降解产物和路径分析，明确功能微生物在降解过程中哪一步起作用，起什么样作用？本项目为揭示“土壤动物-土著微生物”联合体在PAEs污染土壤修复中的协同作用，建立一种蚯蚓驱动的土壤PAEs类塑化剂污染原位修复技术提供理论支持。

Bioremediation technology is the main method to eliminate the PAEs in the field. The low environmental adaptability of exogenous microorganisms seriously limits its practical application. Hence, strengthening native microbial remediation in situ has become a research hotspot.This project uses earthworm repair PAEs in soil. After clear the effect and pathway of earthworm on enhancing PAEs degradation, our project will make unknown metabolic functions of microorganisms and PAEs degradation process linked by stable isotope probing and high-throughput sequencing technology. We lay emphasis on the study of the species composition and evolution of degrading microorganisms in soil, especially the key microorganisms involved in ester bond hydrolysis, excavate degradable microorganism resources. Using the macro transcriptome, we analyze the genes with significant changes in expression during PAEs degradation, and identify the key (new) genes and functions involved in PAEs degradation under earthworm enhancement. Combined with the classical molecular biology technology, its function is verified. Using metagenomic techniques, we want to find out which microbes contain the key genes. Combining metabolites and pathway analysis, we want to know what are the role of functional microbes and how do the functional microbes work? The project is to reveal the important role of " earthworm -microbes "commonwealth in PAEs contaminated soil repaired process, make a theoretical basis and technical support for the development of in situ remediation technologies of soil PAEs.