针对作用于神经系统的有毒中药易引起的中枢神经毒性，文献研究表明白芍常与中枢神经毒性川乌、附子、马钱子等含生物碱类中药配伍，以制约毒性；前期实验表明白芍与马钱子配伍能够降低中枢神经毒性中药马钱子中马钱子碱在脑中浓度。以此类中枢神经毒性中药生物碱类成分为模型药，对白芍活性成分调控此类生物碱类成分透血脑屏障(BBB)作用进行分子对接技术模拟预测；运用MDCK-MDR1细胞模型，对调控透BBB性能进行研究；采用荧光定量PCR、蛋白电泳等技术，研究白芍活性成分与生物碱类配伍对P-gp表达、与药物结合位点的关系、对ATP酶活性及细胞膜流动性的影响；并对白芍调控BBB作用与生物碱类成分结构类型进行关联，阐明白芍调控P-gp介导有毒中药生物碱类成分透BBB的物质基础、作用机制和结构选择关联性。从而为解决中药生物碱类成分在临床上中枢神经毒性提供新思路，为含有毒中药类方研究开发及临床用药安全性提供科学依据。

For those toxicity drugs acting on the nervous system can cause toxic on central nervous system, the literature studies have shown that Radix Paeoniae Alba often combined with medicine containing alkaloid, which have central nervous system toxicity, such as Radix Aconite, Radix Aconiti Lateralis and Strychni semen for restricting toxicity. The preliminary experimental results indicated that Radix Paeoniae Alba could reduce central nervous system drugs strychnine concentration in the brain. Putting this type of central nervous system toxicity herbs alkaloids-containing as model drug, we will simulate and predict the regulation of the active components on action of blood-brain barrier in alkaloids affected by P-gp in Radix Paeoniae Alba at the molecular level. Using MDCK-MDR1cell model, with the technology of FQ-RT-PCR and protein electrophoresis, we could not only study the effect of active substance of Radix Paeoniae Alba on the express of P-gp, P-gp drug binding site, the ATPase activity, the cell membrane fluidity, but also illustrate the material base, the mechanism and structure relevance of Radix Paeoniae Alba on regulating drugs transport that affected by P-gp. The research will provide new ideas to solve the central nervous system toxicity alkaloids of traditional Chinese medicine, and lay a scientific foundation for development on parties in toxic medicine, as well as clinical drug safety.