树突状细胞（DCs）的分化成熟是自身免疫性肝炎(AIH)发病中的重要环节。我们前期发现miR-155调控DCs的成熟，并鉴定了一个新的miR-155相关的反义长链非编码RNA即lncRNA-AADC，沉默lncRNA-AADC可抑制miR-155宿主基因MIR155HG的表达。因而提出“lncRNA-AADC通过改变MIR155HG的转录活性调控miR-155的水平，进而影响DCs的成熟与功能参与AIH发生”。为此，本课题拟重点研究AIH患者和AIH模型小鼠的DCs、体外成熟的DCs中lncRNA-AADC、MIR155HG及miR-155的表达；探索lncRNA-AADC调控MIR155HG的分子机制；靶向DCs干预lncRNA-AADC的表达，观察其对AIH肝损伤程度的影响，以证实上述假设。藉此，丰富和完善AIH的发病机制理论并为其治疗提供全新靶点。

Differentiation and maturation of dendritic cells (DCs) play a key role in the pathogenesis of autoimmune hepatitis (AIH). Our previous work showed that miR-155 regulates maturation of DCs. Moreover, we have identified a new lncRNA associated with DCs (lncRNA-AADC), which is an antisense lncRNA highly related with miR-155. Knockdown of lncRNA-AADC inhibited the expression of miR-155 host gene (MIR155HG). Considering the effects of lncRNA-AADC on miR-155, we hypothesized that "lncRNA-AADC regulates the expression of miR-155 by altering the transcriptional activity of MIR155HG, which affects the maturation and function of DCs involved in AIH". We will firstly detect the expression of lncRNA-AADC, miR155HG and miR-155 in mature and immature DCs both in vivo and in vitro. And then, we will identify the precise molecular mechanisms by lncRNA-AADC to modify the biogenesis of MIR155HG. Finally, the therapeutic potential of lncRNA-AADC in AIH will also be investigated to pave the way for a new targeted therapy. Taken together, the result of this study will provide a new insight into the mechanism of DCs participating in AIH and will facilitate the development of new therapies for AIH.