RB1基因调控鸡脂质代谢的分子机制研究

控制肉鸡体内脂肪的过度蓄积是当前肉鸡育种的重要奋斗目标之一。本课题组长期致力于脂肪组织生长发育机理的研究，在前期鸡体脂性状的QTL定位结果发现，鸡视网膜母细胞瘤基因1（RB1）基因是影响鸡腹脂沉积的重要基因，针对鸡腹脂性状的全基因组选择信号分析结果也发现，RB1基因受到了选择的影响。因此，本研究以鸡RB1基因作为候选基因，鉴定RB1基因影响鸡腹脂沉积的功能性SNP位点；研究该基因在肉鸡高、低脂系间的表达差异；分析RB1基因对脂肪细胞增殖和分化的影响及其作用机制。综合以上结果，初步阐明RB1基因影响鸡脂质代谢的分子机制，进而为鸡乃至其它畜禽的分子育种提供参考。

The aim of the current broiler breeding was to control the over deposition of abdominal fat. Our team has been confused on the study about the mechanism of the adipose tissue growth and development for a long time. In the early study, we mapped the QTL for chicken abdominal fat content on chromosome 1 and found that the RB1 gene was important for abdominal fat deposition. The result of genome wide selection signature analysis indicated that the RB1 gene was a significant selection signature. So the RB1 gene was selected as a candidate gene for adipose development in the current study. In this study, we confused on the indentification of the functional SNPs of RB1 gene for abdominal fat deposition, the expression difference of RB1 gene between the lean and fat lines, the effects and regulatory mechanism of RB1 gene on the proliferation and differentiation of fat cells. These results will preliminary clarified the molecular mechanisms of RB1 gene in chicken lipid metabolism, and thus provide important information for the molecular breeding of chicken and even other livestock.