气候变暖与氮沉降下不同繁殖模式外来入侵克隆植物对天敌采食的适应性对策

生物入侵、气候变暖和大气氮沉降是当今生态学领域热点之一。克隆植物可通过克隆繁殖和克隆生殖进行种群延续与扩散，在全球变化下植物入侵中具有重要贡献。以不同繁殖模式外来入侵克隆植物与共存同属本地克隆及非入侵克隆植物为材料，通过气候变暖（增温）与N沉降（N施加）下，母代与子代生长与繁殖对采食响应的多种对开放天敌和温室天敌添加实验，及不同繁殖模式典型入侵植物种群对长期采食响应的固定样地野外种群跟踪实验与SSR标记分子实验，进行无融合生殖分析、次生代谢与基因表达分析、遗传多样性与遗传变异分析，比较气候变暖和N沉降下不同繁殖模式克隆植物对天敌采食的生长与繁殖响应，采食下不同繁殖模式典型入侵克隆植物的次生代谢变化及相关差异表达基因分析，及长期采食下种群繁殖对策与种群遗传变异，最终阐明气候变暖与氮沉降下不同繁殖模式外来入侵克隆植物对天敌采食的适应性对策，从而为不同繁殖模式克隆植物入侵机理提供理论基础与依据。

Biological invasion, climate warming and nitrogen deposition are the hotspots in the field of ecology. Clonal plants can maintain and expand their populations through clonal propagation and clonal reproduction, which significant contribute to plant invasion under global change. Under climate warming (Simulated warming) and N deposition (N supply), we compare alien invasive clonal plants with co-existing congenetic native clonal plants or non-invasive clonal plants with different reproductive modes (i.e. clonal propagation, clonal reproduction and with both) to enemy herbivory. Response of growth and reproduction of first and second generation in alien invasive clonal plants and co-existing congenetic clonal plants with different reproductive modes to open natural enemies, secondary metabolism and gene expression difference of growth and reproduction of typical clonal plants with different reproductive modes to adding enemies, and difference in reproduction and genetic variation to long-term adding enemies were analyzed by open natural enemies experiments, greenhouse adding enemies experiments and field populations tracking experiments, with apomixis analysis, secondary metabolism and gene expression analysis, genetic diversity and genetic variation analysis. Finally, we can clarify the adaptation strategies of alien invasive clonal plants with different reproductive modes to enemy herbivory under climate warming and nitrogen deposition, which will provide a theoretical data and basis for study on invasion mechanism of alien invasive clonal plants with different reproductive modes.