胶孢炭疽菌是引起炭疽病的一种重要的病原菌，能够侵染大量作物，造成重大的经济损失。目前关于胶孢炭疽菌致病的分子机制研究还不够深入。在前期研究中，我们从芒果胶孢炭疽菌中鉴定了一个与活性氧解毒及致病相关的基因CgAP1，该基因编码一个bZIP型转录因子，其敲除突变体对H2O2更加敏感，在高渗环境下生长缓慢，无法在芒果上致病。本项目拟在前期研究的基础上，对突变体的表型做进一步分析，确定CgAP1的生物学功能及亚细胞定位；利用RNA-Sequence技术进行转录谱分析，明确CgAP1参与的调控网络，分析CgAP1的结合位点；利用基因敲除的方法进一步分析CgAP1调控的部分下游基因的生物学功能，揭示CgAP1调控活性氧解毒及致病的分子机制。研究结果将为胶孢炭疽菌致病的分子机理研究填补新的内容，也为炭疽病的防控提供科学依据。

Colletotrichum gloeosporioides is an important pathogen of anthracnose, which is able to infect numerous crops, causing great economic losses. However, the research on C. gloeosporioides is not enough so far. In a previous study, we identified a gene CgAP1 related to reactive oxygen detoxification and pathogenicity form mango C. gloeosporioides, which encoded a bZIP-type transcription factor. The knockout mutant of CgAP1 was more sensitive to H2O2, growing slowly in hyperosmotic environment, and could not cause disease in mango. On this basis, the project plans to do further analysis of the mutant phenotypes, determining the biology function and subcellular localization of CgAP1. The transcript profile will also be analysed using RNA-Sequence, analyzing CgAP1’s regulatory network and binding sites. The biology functions of downstream genes regulated by CgAP1 will be further studied, revealing molecular mechanisms of CgAP1 regulating reactive oxygen detoxification and pathogenicity. The study will fill new contents for the research on the molecular pathogenesis of C. gloeosporioides, providing scientific basis for the prevention and control of anthracnose.