低雌激素致体温调节紊乱，但机制尚未明确。本项目组前期工作显示，低雌激素大鼠中心体温异常，温度敏感神经核团对温度敏感性降低，且体温调节中枢5-羟色胺浓度、受体数量均增加。本项目拟论证5-羟色胺通路异常致体温调节中枢温度敏感神经元放电频率变化及离子通道异常开闭是低雌激素状态下体温调节紊乱的机制。首先选取低雌激素体温调节紊乱大鼠脑片，测定不同温度下低雌激素对温度敏感神经元自发放电频率及体温调节相关离子通道的影响。第二，阐明5-羟色胺受体在低雌激素体温调节异常大鼠温度敏感神经元的分布特点。第三，根据上述实验结果选取温度敏感神经元上特定5-羟色胺受体亚型激动剂和拮抗剂，观察在低雌激素时，特定受体亚型激动或抑制是否改变温度敏感神经元自发放电频率及相关离子通道开闭。最后，在体内和体外明确雌激素改善低雌激素致温度敏感神经元功能紊乱的效用。本项目旨在阐明低雌激素致下丘脑体温调节中枢功能紊乱的神经递质机制。

Estrogen deficiency is correlated with thermoregulation disorder，of which the mechanism is still unclear. Our preparatory work showed rats with estrogen deficiency had abnormal core temperature and their temperature sensitive nuclei were less sensitive. We also found that with estrogen deficiency, serotonin concentration and the number of serotonin receptors in thermoregulation center were higher. Our project intents to prove that the abnormal change of firing frequency and ion channels opening or closing in the temperature sensitive neurons derived from the serotonin pathway in the thermoregulation center is the mechanism of the temperature regulation disorder in estrogen deficiency condition. First, the brain slices of rats which have disordered temperature regulation in estrogen deficiency condition will be selected, the effect of estrogen deficiency on spontaneous firing rate and the thermoregulation relevant ion channels of temperature sensitive neurons will be measured. Then the distribution of 5-hydroxytryptamine receptors on temperature sensitive neurons will be shown in rats which have abnormal temperature regulation related to estrogen deficiency. Third, certain subtypes of 5-hydroxytryptamine receptors on temperature sensitive neurons will be excited or blocked according to the experiment above and see if the spontaneous firing rate and the opening or closing of thermoregulation relevant ion channels change in estrogen deficiency condition.Finally, the utility of estrogen in vivo or in vitro on the disorder of temperature sensitive neurons derived from estrogen deficiency. We aim to explain the mechanism of estrogen deficiency-induced disorders of hypothalamus in temperature regulation and provide new ideas for treatment.