肿瘤特殊微环境在肿瘤转移中发挥重要作用，我们前期证实缺氧是促进胃癌转移的关键因素，但机制尚不清楚。LncRNA是一类长度大于200nt的非编码RNA，可从多个角度调节蛋白编码基因活性，是当前肿瘤研究热点。我们首次用高通量lncRNA芯片进行了缺氧诱导的胃癌细胞以及高转移胃癌组织、细胞中的lncRNA的差异筛选，发现lncRNA-uc341在缺氧诱导以及胃癌高转移的组织和细胞中均上调。功能学实验证实了其在缺氧诱导胃癌细胞转移中发挥重要作用。进一步的生物信息学分析以及文献回顾发现其定位于转移相关靶基因HOXC10附近，可能通过甲基化作用发挥其调控机制的。本项目拟借助RT-PCR,CHIP，EMSA、启动子甲基化等技术进一步探讨缺氧条件下其对HOXC10的转录调控机制，以揭示lncRNA-uc341-HOXC10在缺氧诱导胃癌转移中的作用机制，为临床阻断转移提供新思路。

It is now becoming clear that the tumour microenvironment (e.g: hypoxia) is an indispensable participant in the cancer metastasis.We initially confirm that hypoxia is a key factor to promote the migration and invasion of gastric cancer cells. However, the mechanism still remains unclear.Long non-coding RNAs (lncRNAs) are a novel class of non-coding transcripts, which are more than 200 nucleotides in length and involved in the regulation of gene expression in various ways.It has been a popular topic in tumor study.Differentially expressed lncRNAs between gastric cancer cells exposed to hypoxia and normoxia; normal human gastric epithelia,primary gastric carcinomas, and distant metastatic tissues and cell lines were identified by microarray . LncRNA-uc341 expression was markedly upregulated in gastric cancer cell lines exposed to hypoxia compared with normoxia and also overexpressed in distant metastatic tissues and cell lines compared with normal's and non- metastatic's by RT-PCR.Here, we demonstrated the role of lncRNA-uc341 in hypoxic metastasis and invasion in gastric cancer.Further bioinformatics analysis and systematic literature review, we identify its location in the chromosomes coincides with the metastases-associated target gene- HOXC10,may play its regulation mechanism by DNA methylation.This project will further explore the mechanism of how lncRNA-uc341 mediate HOXC10 in hypoxia condition by RT-PCR, CHIP, EMSA, DNA methylation techniques.To reveal the mechanisms of lncRNA-uc341-HOXC10 contributes to hypoxia-induced invasion in gastric cancer, and provide a new thinking of reversing tumor metastasis in clinical.