血管内皮细胞的粘附和迁移是心血管材料植入后内皮化修复的重要过程。通过材料表面调控细胞粘附、迁移等生物学行为，是当今细胞生物力学研究的焦点，但其分子机制尚未明确。本项目拟采用SAMs技术制备不同化学官能团（CH3、NH2、COOH、OH）的模式材料表面，分析不同材料表面化学对血管内皮细胞粘附和迁移的影响。在此基础上，通过对ECM-Integrins-FAs-FAK-Rho GTPases这条与细胞粘附迁移密切相关的信号轴上关键蛋白表达，分析其在细胞内的分布、募集活化的时序规律以及对粘附摩擦力和牵引力的贡献等，从"细胞－分子－力学"不同层次探寻材料表面化学调控细胞粘附和迁移的作用规律及其生物力学机制。本项目研究结果将在材料表面性质调控细胞粘附/迁移行为、关键蛋白表达以及细胞的生物力学特性之间建立一条联结纽带，有助于从新的角度去认识细胞与材料的表/界面行为，揭示细胞识别材料表面特征的分子机制。

The adhesion and migration of vascular endothelial cells (VECs) are the essential processes of reendothelialization after implantation of cardiovascular materials. The surface of materials could regulate adhesion and migration behaviors of VECs, which is a research focus of cellular biomechanics. However, the molecular mechanism is unclear. In this project, the modified surfaces with different chemical functional groups (CH3, NH2, COOH, OH) by self-assembled monolayers (SAMs) would be used to examine the effect of various surface chemistry on VECs adhesion and migration. In addition, the expression levels, distrubution, clustered and activated sequence of critical proteins in ECM-Integrins-FAs-FAK-Rho GTPases pathway associated with cell adhesion and migration would be studied to explore the biomechanical mechanism of surface chemistry regulating VECs adhesion and migration in "cells-molecules-mechanics" three levels. The finding of this project would help us to understand the interrelationship among surface properties regulating cell behaviors, crucial protein expression and cellular biomechanical characteristics, to know the behavior of surface/interface between biomaterials and cells, to reveal molecular mechanism of cells sensing surface characterization.