组织工程膀胱重建具有重要的临床应用价值。本课题组成功构建了复合型功能修复材料，在膀胱脱细胞基质材料（BAM）上成功交联碱性成纤维细胞生长因子（bFGF），建成了CL-bFGF/BAM组织材料，在小面积膀胱组织修复中有较好的治疗作用，但大面积组织修复中效果不理想，导入具有自我复制和分化功能的种子细胞可能是重要的解决策略。据此，我们前期成功分离及鉴定出了尿源干细胞(USCs)，并建立了向膀胱组织细胞分化的技术方案。本项目拟将USCs导入CL-bFGF/BAM材料，利用材料中交联的bFGF激活其与组织修复相关的丝裂原活化蛋白激酶（MAPK）和基质金属蛋白酶（MMP）的表达及功能，促进USCs向膀胱组织的分化。在观察其修复膀胱的基础上，进一步通过阻断MAPK及MMP通路，探讨CL-bFGF/BAM材料促进USCs膀胱修复中的作用机制，为临床上膀胱重建提供新的策略和方法。

Tissue engineering for bladder reconstruction has great clinical value. Our previous study successfully constructed functional composite materials, cross-linking(CL) basic fibroblast growth factor (bFGF) into the bladder acellular matrix material (BAM) to construct CL-bFGF/BAM, It was effective in the small area of the bladder tissue repair, while not effective in the large area of tissue repair. the import of seed cells may be important to solve this problem. Accordingly, we have successfully isolated and identified the urine-derived stem cells (USCs), and established the protocol of USCS differentiation toward into bladder tissue cells.This project intends to import USCs into CL-bFGF/BAM material, the cross-linked bFGF activates mitogen-activated protein kinase (MAPK) and matrix metalloproteinase (MMP) pathway, promotes USCs proliferation and differentiation. By blocking the MAPK and MMP pathway, the mechanism of CL-bFGF/BAM promoting USCs bladder repair will be explored, providing new methods and strategies for bladder reconstruction