舟山海域岛屿众多，鱼类生物多样性丰富，由于传统调查手段限制，长期以来缺乏快速、有效的方法对其进行准确评估。环境DNA metabarcoding技术是一种低耗、高效、高灵敏度的物种监测新技术，近年来，已成为监测生物多样性的主要手段。本研究拟以舟山近海为研究区域，旨在：（1）建立一套针对近海河口的高质量环境DNA提取方法；（2）设计条形码基因通用引物，通过高通量测序和metabarcoding分析，进行舟山近海鱼类的准确鉴定；（3）结合渔业资源调查，明晰舟山近海鱼类多样性及其时空分布；（4）开展褐菖鲉不同密度养殖实验，建立环境DNA浓度与其生物量之间关系模型，并进行验证。本项目不仅可以建立基于环境DNA metabarcoding技术的近海鱼类生物多样性评估方案，揭示舟山海域鱼类多样性及其时空变化规律，同时可为海洋生物资源调查研究提供参考依据和技术支撑，具有重要的理论意义和应用前景。

Biodiversity is of great value to human being and is an important indicator of ecosystem responding to environmental change and human activities. Zhoushan coastal area, is abundant in high fish diversity and fisheries, which is determined by traditional trawl net sampling and in need of a modern and accurate information. Environmental DNA (eDNA), characterized by low cost, high efficiency and sensitivity, is becoming more and more popular in monitoring biodiversity. Focused on Zhoushan costal area, this study intends to (1) Establish a high-quality environmental DNA extraction method; (2)Design universal primers of barcoding genes and identify accurately on fish species based on NGS (Next Generation Sequencing); (3) Explain fish diversity and the spatial-temporal distribution pattern based on seasonal investigation; (4) Establish a biomass assessment model according to eDNA concentration of Sebastiscus marmoratus in laboratory experiments with different density gradients. We expected the outputs of a universally eDNA-based evaluation scheme for estuarine fish diversity, and an explanation of fish diversity and distribution in Zhoushan coastal area. This could supply a scientific reference and technical support for an efficient resource and sustainable fisheries.