miR158调节油菜作物响应非生物胁迫作用机理的研究

miRNA是一类植物中独特的小分子RNA，它在调控植物逆境胁迫中发挥重要作用。最近，申请人通过高通量测序鉴定了油菜中的miR158及其靶基因——RNA解旋酶(DEAD-box ATP-dependent RNA helicase 6 )。初步研究表明，油菜miR158受镉，盐和冷害等非生物胁迫的诱导表达，而其靶基因BnaA04g26450D和BnaC04g50480D在镉等非生物胁迫下表达下调，并且相关报道表明RNA解旋酶在非生物胁迫中发挥重要作用。本项目拟通过构建miR158和及其靶基因的过表达载体，转化获得油菜转基因植株，探索miR158及其靶基因在镉等非生物胁迫下的表达模式、转基因植株的表型变化、生物量的改变及其在重金属镉等非生物胁迫下的生理生化反应。本项目立足于研究油菜miR158受镉等非生物胁迫的分子机理，同时将进一步探索油菜中miR158及其靶基因在非生物胁迫下所发挥的作用。

miRNAs are a distinct class of small RNAs in plants that play an important role inthe regulation of plant environment stresses. Recently, we identified miR158 and its target DEAD-box ATP-dependent RNA helicase 6, from Brassica napus. Our previous experiments have demonstrated that miR158 was up-regulated in expression under Cd, NaCl and Cold stresses in Brassica napus, and its targets BnaA04g26450D and BnaC04g50480D was down-regulated in expression under these abiotic stresses. In addition, it was reported RNA helicases play an important role in abiotic stresses. In this study, we will construct overexpression vectors of the miR158 and and its target and transform them into Brassica napus, respectively. The expression patterns of miR158 and its target, the characteristics of phenotype, biomass and mechanisms of physiology and biochemistry will be studied under heavy metal Cd stress in the control and transgenic plants. The study is based on the molecular mechanism of miR158 induced by cadmium. The results also may provide the evidences in the role of miR158 and its target under Cd stress in Brassica napus.