多环芳烃（PAHs）是一类具有"三致"效果的高毒性有机化合物，长期低浓度环境暴露，对细胞和DNA造成损伤,从而引起各种严重的慢性呼吸道疾病。研究以广州市患有各种慢性呼吸道疾病的儿童为对象，以尿中12种单羟基多环芳烃作为PAHs短期暴露的标志物,测定儿童体内PAH水平，分析其特点并评估其环境风险；以尿中8-羟基脱氧鸟苷和血中特异性的7，8-二醇-9，10-环氧苯并芘（BPDE）-DNA加合物为标志物评价PAHs长期暴露对DNA的损伤；用肺功能和血清中白细胞介素-作为一个早期效应指标评价PAHs污染暴露对呼吸系统的不良影响。找寻和鉴定PAHs暴露对肺功能和呼吸道系统损伤最灵敏和适用的生物标志物。并结合大气资料和生活饮食习惯，从尿中PAHs代谢产物和DNA加合物角度综合评价PAHs典型城市环境污染对儿童健康的潜在危害，建立数学模型对PAHs内暴露与肺功能和DNA损伤进行量化描述,具有明显创新性。

Polycylic aromatic hydrocarbons (PAHs) are the carcinogenic compounds with high toxicity. Even the long time exposure to low level will lead to DNA damage and chronic respiratory disease, especially for children due to their sensitivity. As Guangzhou ranked 5 in 14 cities with high environmental PAHs environment in China, especially in winter,the local residents may be exposure to PAHs with high environmental risk. Our objectives of this study is to investigate the dose -effect related relationship between carcinogenic target compounds and their DNA damage, as well as PAH exposure effects on lung function for children living in Guangzhou. Children who has chronic respiratory disease (such as bronchial asthma,chronic obstructive pulmoary disease, pneumonia et al) with the age between 6-11 years old in Guangzhou are recruited，and the healthy children in Conghua were recruited as the control group. 12 urinary hydroxylated PAH metabolites are selected as the biomarkers to assess the short-term exposure levels to PAHs. The profile levels and characterization of PAHs exposure will be fully discussed and the environment risk of PAHs exposure will be assessed by using urinary PAHs exposure. 8-hydoxy-2'-deoxyguanosine (8-OHdG) in urine and specific Benzo(a)pyrene [B(a)P] diolepoxide (BPDE)-adduct in peripheral blood are used respectively as the short and long-term biomarkers to evaluate the effects of PAHs on DNA damage, as well as the environmental risks. The method of 32p postlabelling coupled with liquid chromatography to quantify the BPDE-DNA adduct in blood will be developed and standardized. Chromatographic method pretreated with multiple clean-up procedures may lower the detection limit of BPDE-DNA adduct. The dose-response associations between PAHs exposure and DNA damage and the most sensitive biomarker to assess the PAHs exposure will be investigated. Lung function and serum interleukin-8 (IL-8) are used as the early biomarkers o effect to assess the PAH pollution on respiration system. Furthermore, based on the suitable and sensitive variables, factor analysis will be used to set up a quantitation model to discript the association beween urinary PAHs exposure and DNA damage, lung function. In one word, the study is scientifically significant and a combination of analytical chemistry and toxicology in the employment of environment science. The combination and employment of epidemiology, statistics, analytical chmistry and mathematical model in environment science will give a profile assessments of the PAHs exposure on children's respiration health hazards and broaden the research depth.