从mTOR/S6K1信号通路探讨健脾化湿方改善2型糖尿病胰岛素抵抗的分子机制

胰岛素抵抗是2型糖尿病的显著特征。本项目在我们前期工作观察到健脾化湿方能有效改善胰岛素抵抗的基础上，拟复制2型糖尿病胰岛素抵抗动物模型，用健脾化湿方进行干预，观察大鼠血脂、血糖、胰岛功能以及骨骼肌mTOR、S6K1、PGC-1α mRNA及蛋白表达，以论证"脾虚痰湿是2型糖尿病胰岛素抵抗的重要发病机理，健脾化湿方调节糖脂代谢改善脾虚痰湿型2型糖尿病胰岛素抵抗的机制之一可能是通过调节mTOR/S6K1信号通路实现"的假说，对阐明健脾化湿方改善2型糖尿病胰岛素抵抗的分子机制，丰富"脾主运化"的理论的内涵并指导临床实践具有重要意义。

Insulin resistance is a significant feature of type 2 diabetes. On the basis of our previous work(strengthen spleen resolving dampness decoction can effectively improve insulin resistance) ， this project will replicate animal models of insulin resistance in type 2 diabetes. With the intervention of strengthen spleen resolving dampness decoction, serum lipids, blood glucose, islet function, skeletal muscle of mTOR of S6K1, PGC-1α mRNA and protein expression will be observed before and after treatment in order to demonstrate that spleen hypofunction and dampness accumulation is a important pathogenesis of insulin resistance in type 2 diabetes. One of the mechanisms which strengthen spleen resolving dampness decoction can regulate glucose and lipid metabolism and improve insulin resistance in type 2 diabetes (spleen hypofunction and dampness accumulation type) may be achieved by regulating mTOR/S6K1 signaling pathway. It is very important for clarifying the molecular mechanisms which strengthen spleen resolving dampness decoction can effectively improve insulin resistance in type 2 diabetes, enriching the theoretical connotation of spleen controlling digestion, guiding clinical practice.