水牛肌内脂肪沉积关键基因的挖掘

我国牛肉持续供不应求，向资源丰富的水牛要牛肉是缓解当前国内牛肉供应压力、减少水牛遗传资源流失的一个重要举措。然而，水牛肌内脂肪（intramuscular fat，IMF）沉积能力低下，却又缺乏可用于肉牛IMF沉积改良的有效分子标记。因此，挖掘水牛IMF沉积的关键基因已成为揭示水牛IMF沉积机制、进行水牛肉品质改良的关键。本研究以水牛IMF含量显著低于黄牛为切入点，拟采用高通量测序技术和生物信息学分析方法筛选水牛与黄牛IMF差异表达基因，获得潜在的影响水牛IMF沉积候选基因；以水牛原代脂肪细胞为材料，通过超表达和干扰技术，深入分析候选基因对水牛脂肪沉积效应的影响，以期挖掘出水牛IMF沉积的重要基因3-5个，为进一步揭示水牛IMF沉积机制、促进水牛肉品质改良提供重要理论基础。

Beef is in short supply consistently in our country. Based on the fact that buffalo is considerable rich in China, asking buffalo for meat become an important measure relieving the stress of supply in beef and reducing the loss of buffalo genetic resources. However, the poor intramuscular fat (IMF) in buffalo as well as the absence of any effective molecular marker for beef breeding make the exploration of key genes hindering the deposition of IMF a key step for revealing mechanism of IMF deposition and meat quality improvement in buffalo. Based on significant difference of IMF% between buffalo and cattle, the project will screen differential expression genes between IMF tissue of buffalo and cattle through high throughput sequencing and bioinformatics analysis; reveal their effect on IMF deposition by over expressing and interfering candidate genes in the primary adipocytes of buffalo. Three to five key genes hindering IMF deposition in buffalo will be identified, which provide a significant information for further study on the mechanism of IMF deposition and meat quality improvement in buffalo.