强光诱导多肽(HLIP)是蓝细菌在强光下存活所必需的蛋白质家族。利用光合模式生物蓝藻Synechocystis sp. PCC6803为实验材料，通过转录组起始位点分析、铁稳态上位分析、光氧化胁迫和生理生化分析等方法，以期获得HLIP及相关调控因子pfsR、dspA功能。此外，利用9XHis （或HA, c-myc tags)标记的HLIP 和Slr1128 菌株，大规模分离HLIP-PS I 三聚体和新型胡萝卜素蛋白复合体，并进行蛋白组分鉴定、生理生化特性和光学特性等分析。结果将进一步阐释HLIP功能及其与光合作用和应激反应的相互关系。这三个方面的研究有机结合，不仅有助于解析蓝藻强光诱导多肽的功能还能够揭示蓝藻在强光下的存活的分子机制，并为蓝藻在光合作用的研究、生物能源及保健品开发应用奠定了基础。

High light-inducible protein (HLIP) family is essential to survival of cyanobacteria during exposure to high light. In order to know more about the functions of HLIP and related regulators pfsR and dspA, transcription start site analysis, iron homeostasis analysis, photo-oxidative stress and physiological and biochemical characterization will be performed on the photosynthetic model organism Synechocystis sp. PCC 6803. Moreover, stains of HLIP and Slr1128 tagged with 9XHis （or HA, c-myc tags) will be used to isolate the HLIP-PS I trimer complex and a novel carotenoid-binding complex at large scale. The identity of the protein components, physiological/biochemical characterization and the spectroscopic characteristics will be determined. The information obtained will further elucidate the function of HLIP,and its relation to photosynthesis and stress responses. The combination of the three research objectives will not only clarify the function of HLIP, but also reveal the molecular mechanisms of survival employed by Synechocystis sp. PCC 6803 during exposure to high light. The research also lays important foundation for developing cyanobacteria for various applications in biofuel and nutriceuticals, as well as for photosynthesis research.