Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp. PCC6803.
Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp. PCC6803.
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DOI：
10.1186/1754-6834-7-32
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发表时间：
2014-03-01
影响因子：
6.3
通讯作者：
He Q
中科院分区：
文献类型：
--
作者：
Chen G;Qu S;Wang Q;Bian F;Peng Z;Zhang Y;Ge H;Yu J;Xuan N;Bi Y;He Q
多不饱和脂肪酸（PUFA）在其主链中含有两个或更多个双键，由于其营养价值，医药应用和作为生物燃料的潜在用途，是全球研究的重点。然而，生产这些经济上重要的化合物的能力是有限的，因为从天然油中分离ω-3多不饱和脂肪酸（ω-3 PUFA）既昂贵又具有技术挑战性。虽然一些植物和微藻ω-3多不饱和脂肪酸的生物合成途径已被破译，但目前对集胞藻PCC 6803脂肪酸脱氢酶含量与脂肪酸合成之间的关系的理解是不完整的。我们构建了一系列在光合作用psbA 2启动子控制下的Δ6和Δ15脂肪酸去饱和酶在转基因集胞藻PCC 6803中内源和外源表达的同源载体。我们从集胞藻PCC 6803、藤仓赤霉菌和高山被孢霉中获得了6个同源重组子，它们含有不同的脂肪酸去饱和酶基因。这些品系在20°C和30°C下产生高达8.9 mg/l的α-亚麻酸（ALA）和4.1 mg/l的十八碳四烯酸（SDA），这是相应野生型水平的六倍多。因此，Δ6和Δ15脂肪酸去饱和酶的转基因表达增强了集胞藻属物种PCC 6803中特异性ω-3 PUFA的积累。在蓝绿集胞藻（Synechocystis sp. PCC 6803）中，外源和内源PUFA脱氢酶基因的过量表达显著增加了ALA和SDA的积累，降低了亚油酸和γ-亚麻酸的积累。这项研究为增加蓝藻的脂肪酸含量奠定了基础，并最终为生产具有高水平必需ω-3 PUFA的营养和医药产品奠定了基础。
Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (ω-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal ω-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete. We constructed a series of homologous vectors for the endogenous and exogenous expression of Δ6 and Δ15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9 mg/l of α-linolenic acid (ALA) and 4.1 mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20°C and 30°C. Thus, transgenic expression of Δ6 and Δ15 fatty acid desaturases enhances the accumulation of specific ω-3 PUFAs in Synechocystis sp. PCC6803. In the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and γ-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential ω-3 PUFAs.
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DOI：
10.1073/pnas.0511079103
发表时间：
2006-06-20
影响因子：
11.1
作者：
Damude, Howard G.;Zhang, Hongxiang;Yadav, Narendra S.
通讯作者：
Yadav, Narendra S.
影响因子：
7.1
作者：
Calder, Philip C.
通讯作者：
Calder, Philip C.
影响因子：
7.4
作者：
Bouaid, Abderrahim;Martinez, Mercedes;Aracil, Jose
通讯作者：
Aracil, Jose
影响因子：
7
作者：
Calder, Philip C.
通讯作者：
Calder, Philip C.
影响因子：
6.3
作者：
Lei A;Chen H;Shen G;Hu Z;Chen L;Wang J
通讯作者：
Wang J