循环肿瘤细胞(CTC)是外周血中自由循环的癌细胞,CTC活性监测在疗效评估、药效分析、个性化治疗等方面具有重要作用；然而，传统方法无法保持CTC的活性,无法满足现代生物医学研究对CTC监测的需要。因此，研究新型CTC实时在线检测体系具有很好的实际意义。 本项目将光寻址电位传感器(LAPS)的表面场效应敏感机制、石墨烯类材料优良电化学性能、核酸适配体(简称适配体)捕获靶CTC的生物识别过程相结合，提出了基于石墨烯增强-适配体-LAPS阵列的CTC实时在线检测体系。本项目的特点在于：1）与传统LAPS器件相比，石墨烯增强了响应信号的强度；2)以适配体作为CTC的探针具有较高的靶向性和亲合力；3）LAPS阵列与流体芯片相结合，可实现CTC多靶向点、实时在线检测。 本项目即可满足生物医学研究的当前需要，又可为细胞学研究提供新的实验技术方法，具有较好的实际意义和科学价值。

Circulating tumor cell (CTC) is a kind of malignant tumor cells circulating freely in the peripheral blood. CTC's livingly detection is very important to assess curative effect of a treatment, to analyze a medicine's effect , or to map out a personalized therapy program, etc.. While the activity of CTC can not be maitained by the traditional detection methods, the requirement to monitor CTC livingly in many modern biomedical researches can not be fulfiled. So it is of great significance to develop a realtime and online detection system of CTC. In this proposal, a realtime and online CTC's detection system is suggested based on the combination of the surface electrical field sensing mechanisim of LAPS (light addressable potentiometric sensor), the excelent electrochemical characteristics of the chemically derived graphene material and the speicific biological identification process between the aptamer and its target CTC. The merits of this proposal consist in three aspects which are: 1) compared with traditional LAPS, intensity of responding signal could be enhanced with the help of chemically derived graphene materials; 2) high specificity and affinity are possessed by aptamers which are used as CTC's probes at here; 3) By the integration of the micorfluidic technique and LAPS' array, CTC's multi-taget online and realtime detection could be realized. The fulfillment of this proposal could not only meet the current requirements for CTC online and realtime detection, but also provide a novel experimental technique and means for the research of cells in other biological system. It is believed that this proposal is worthy of being funded because of its practical and scientific value for the biomedical and cytological applicaitons.