采用多基因转化技术（整合至核基因组）和多基因质体转化技术（整合至叶绿体基因组），并以不同的代谢改造方式（乙醇酸 至 乙醛酸 至 草酸 至 CO2；乙醇酸 至 乙醛酸 至 羟基丙酮酸半醛 至 甘油酸 + CO2），在水稻叶绿体中分流光呼吸乙醇酸并释放CO2于其中，以此提高Rubisco周围的CO2浓度、创建水稻光合CO2浓缩机制（CCM）。依据相关报道与本课题组的前期研究基础，可望由此一方面提高植物光合效率并减少CO2释放，另一方面可望深入阐明光合效率与水稻产量形成的关系及其机理，还可能由此改善植物的抗逆能力（干旱、高温、高光等）。因此，本项目研究对深入阐明高光效机理、对作物生产、甚至对减少大气碳排放均具有深远意义。

In this project, we aim to construct the photosynthetic CO2 concentrating mechanism (CCM) in rice plants through increasing the ambient CO2 concentration surrounding Rubisco by releasing the photorespiratory CO2 within the chloroplast. The metabolic engineering will be carried out by using both multigene transformation (integrated into nuclear genome) and multigene plastid transformation (integrated into chloroplast genome) approaches to bypass the photorespiratory glycolate into CO2 in different ways, i. e. glycolate to glyoxylate to oxalate to CO2; glycolate to glyoxylate to tartronate semialdehyde to glycerate+CO2. Based on the related literature and our own pioneering investigations, this engineering would be able to improve photosynthetic efficiency meanwhile lowering CO2 loss in plants, and then to in-depth understand the relationship between photosynthetic efficiency and crop yield formation and its mechanism, and also hopefully, to increase stress resistance of rice (drought, heat and high light, etc.). Therefore, this study is of both academic and practical significance in terms of understanding the mechanism of high photosynthetic efficiency, its relations to crop yield formation, and even of manipulating carbon emissions in the atmosphere.