TGF-β1在ALI/ARDS 发病机制中起非常重要作用，我们发现curcumin能够有效治疗脓毒症性ALI，部分作用可通过影响TGF-β表达实现。本项目以脓毒症性ALI为研究对象，以运用计算机预测和miRNA 芯片筛选发现的与之相关的调控TGF-β1基因的miRNA为切入点，通过构建表达质粒，在细胞水平鉴定、筛选能下调TGF-β1表达的特征性miRNA 并合成反义抑制剂进行确证。检测curcumin 对该 TGF-β1 mRNA 和蛋白表达的影响，并在动物模型上验证miRNA分子表达与TGF-β1表达相关性，从基因易感性层面揭示其作用。本研究将可能证明curcumin 通过调节miRNA 表达，从而下调TGF-β作用，因此干预脓毒症性ALI 过程这一科学问题，也可能为脓毒症性ALI 治疗研究提供新的靶点和切入点。

TGF-beta 1 plays an important role in the pathogenesis of ALI/ARDS, we found that curcumin can effectively treat septic ALI, some of the effects can be achieved by affecting the expression of TGF-beta. This project takes septic ALI as the research object, by the use of computer prediction and miRNA chip for screening found associated with the regulation of the TGF-beta 1 gene expression plasmid miRNA as the starting point, through the construction, in the screening of cell level identification, down-regulation of TGF-beta 1 expression characteristics of miRNA and the synthesis of antisense inhibitors confirm. The effect of curcumin on the expression of TGF-beta 1 mRNA and protein was detected, and the expression of miRNA and the expression of TGF-beta 1 was verified in animal model. This study may prove curcumin through regulating the expression of miRNA, and down-regulation of TGF-beta, so the intervention of a problem on septic ALI process, may also provide a new target and starting point to study the treatment of sepsis in ALI.