农用和医用抗生素的广泛滥用导致大量耐药菌的产生和传播。已有的报道证实机体内非编码RNA与细菌的抗逆性和耐药性密切相关。在前期的研究中，申请者获得一株对多种抗生素均有耐药性的土霉素降解菌株Arthrobacter nicotianae ZAF-05，全基因组测序分析表明该菌株包含23个与抗生素抗性相关的基因以及多条功能未知的非编码RNA序列。本项目拟采取以下研究以期以不同于蛋白质编码基因的角度阐述sRNA在菌株ZAF-05对多种抗生素发挥耐药性的作用机制： 1）采用生物信息学预测鉴定菌株ZAF-05中全部sRNAs；2）基于预测数据，利用芯片技术对抗生素胁迫条件下菌株的sRNA转录谱进行差异分析，挖掘耐药性相关sRNA；3）最后通过基因敲除、全转录谱分析、sRNA靶标分析等手段研究这些相关sRNA在菌株耐药过程中的作用机制。实验结果对于填补由sRNA介导的抗生素耐药机理研究空白具有重要意义。

The abuse of antibiotics in clinical and agricultural sectors is causing the fast emergence and spread of bacteria with antibiotic resistance. An oxytetracycline degrading strain Arthrobacter nicotianae ZAF-05, with multiple antibiotic resistances was obtained in previous studies. Genome sequencing analysis showed that the strain contains 23 antibiotic resistance-related genes and many function-unknown sRNAs. The project aims to elaborate novel regulation mechanisms of sRNAs in its tolerances to antibiotics, which are different from the regulation mechanisms controlled by protein-coding genes by taking the following studies: 1) to screen sRNAs in Arthrobacter nicotianae ZAF-05 by bioinformatic predictions; 2) to isolate sRNA related to antibiotic resistence by using chip technology to analyze sRNA transcriptional profiling differences in Arthrobacter nicotianae ZAF-05 grown under different antibiotics pression; 3) to illuminate the action mechanisms of target sRNA under antibiotic process by gene knockout, full transcriptional profiling analysis, and so on. The study will fill the research gaps in the field of sRNA-mediated antibiotic resistance mechanism.