Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16.
Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16.
复制标题
DOI：
10.1038/s41598-021-94840-y
复制
发表时间：
2021-08-02
影响因子：
4.6
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Zhang X;Zhu R;Li W;Ma J;Lin H
虽然许多细菌有可能从环境中去除抗生素残留，但使用抗生素降解细菌来管理抗生素污染的好处应该针对抗生素耐药性潜在扩展的风险进行评估。本研究调查了细菌烟草节杆菌OTC-16的抗生素耐药性模式，其显示土霉素（OTC）/四环素的大量生物降解。结果表明，该菌株对15种抗生素中的至少7类抗生素具有耐药性。A.烟草赤霉OTC-16含有一条染色体（3，643，989 bp）和两个质粒（质粒1，123，894 bp和质粒2，29，841 bp）。在分离出的3,561个基因中，有8个与抗生素耐药性有关。在菌株OTC-16降解OTC期间，使用实时定量逆转录-聚合酶链反应（qRT-PCR）追踪质粒中ant 2 ia、sul 1、tet 33和cml_e8以及染色体中一个基因（tetV）的表达。只有质粒来源的耐药基因在土霉素的存在下上调。土霉素的存在增加了菌株OTC-16对硫酸链霉素的耐受性。本研究的发现有助于加深我们对耐药基因行为特征和耐药菌适应性进化的理解。
Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic-degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC-16, which shows substantial biodegradation of oxytetracycline (OTC)/tetracycline. The results showed that this strain could be resistant to at least seven categories of 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC-16 contains one chromosome (3,643,989 bp) and two plasmids (plasmid1, 123,894 bp and plasmid2, 29,841 bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During OTC degradation by the strain OTC-16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene (tetV) in the chromosome were tracked using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only the plasmid-derived resistance genes were up-regulated in the presence of OTC. The presence of OTC increased the tolerance of strain OTC-16 to streptomycin sulphate. The findings of this study can help deepen our understanding of the behavioural characteristics of resistance genes and adaptive evolution of drug-resistant bacteria.
登录
查看更多内容
影响因子：
14.9
作者：
Kanehisa M;Furumichi M;Sato Y;Ishiguro-Watanabe M;Tanabe M
通讯作者：
Tanabe M
影响因子：
11.4
作者：
Deng, Yu;Wang, Yulin;Zhang, Tong
通讯作者：
Zhang, Tong
影响因子：
4
作者：
Ghosh, S.;Sadowsky, M. J.;LaPara, T. M.
通讯作者：
LaPara, T. M.
影响因子：
15.5
作者：
Marathe, Nachiket P.;Berglund, Fanny;Larsson, D. G. Joakim
通讯作者：
Larsson, D. G. Joakim
DOI：
10.13227/j.hjkx.202001081
发表时间：
2020-08-15
期刊：
Huanjing Kexue
影响因子：
--
作者：
Gao Pin;Ruan Xiao-hui;Qian Ya-jie
通讯作者：
Qian Ya-jie