基于局域表面等离子体共振（LSPR）技术的生物传感器具有灵敏度高、特异性好，无需标记、检测速度快、易集成等优点，已应用在外泌体传感检测领域。然而，目前基于LSPR技术的外泌体检测传感芯片制备成本高、利用单一抗体检测造成外泌体亚群丢失，限制了该技术在外泌体检测中的应用。本项目通过发展基于LSPR技术的传感芯片制备技术，解决以上外泌体检测难题。利用阳极氧化铝（AAO）薄膜制备多阵列金纳米颗粒传感单元，与多通道微流控芯片集成，制备低成本、高检测重复性的LSPR传感芯片；以外泌体为研究对象，通过膜联蛋白V功能化金纳米颗粒表面，靶向外泌体表面磷脂酰丝氨酸，再利用多种特异性抗体识别所捕获的外泌体，检测外泌体亚群含量。最终，本项目低成本、高重复性多阵列LSPR-微流控集成芯片的开发可推动局域表面等离子体共振技术在外泌体检测领域中的发展和应用

The biosensor based on localized surface plasmon resonance (LSPR) technology has the advantages of high sensitivity, good specificity, label-free, fast, easy to integrate and so on. It has been used in the detection of exosomes. However, at present, the exosome detection biosensor chip based on LSPR technology has high cost, and the loss of exosome subpopulation caused by single antibody detection, which limits the application of this technology in exosome detection. In this project, LSPR based biosensor chip preparation technology is developed to solve the above problems in exosomes detection. The multi-array Au nanoparticles sensor unit was fabricated by using AAO thin film and integrated with multi-channel microfluidic chip to prepare LSPR sensor chip with low cost and high detection repeatability. The exosomes were studied by functionalizing gold nanoparticles with annexin V and targeting phosphatidylserine on the surface of exosomes. The exosomes were identified by a variety of specific antibodies to detect the subpopulation of exosomes. Finally, development of the multi-array LSPR microfluidic integrated chip with low cost and high repeatability can promote the development and application of LSPR technology in the field of exosomes detection.