早熟性是油菜育种的重要目标性状之一，种子成熟早晚是由开花期决定的。miR169家族在植物的生长发育过程调控起重要作用。在miRBase中，油菜 Bna-miR169 家族有 16个成员，但Bna-miR169 家族基因的功能均未见报道。我们的前期研究表明：过量表达Bna-miR169d能促进油菜早开花早熟，与前人在拟南芥中的研究结果一致。本项目拟在已有工作基础之上鉴定并克隆Bna-miR169d的靶基因，分析该类基因在植株体内的表达模式；构建靶基因过表达和靶基因RNAi 载体转化油菜，通过对不同转化体及野生型植株进行表型、细胞、生理和基因转录水平的对比研究，分析Bna-miR169d及其靶基因在油菜早熟发育中的作用，为进一步阐明Bna-miR169d调控早熟发育的分子机制奠定基础，为早熟油菜种质的创制及生产应用提供依据和基因资源。

Early maturity is one of the important traits in rapeseed production.Blossom period is an important index of the maturity traits.The miR169.family play important roles in the regulation of plant growth and development.16 members of miR169 family in rapeseed have been registrated in current miRBase.The studies about cloning and function of miR169 family genes have not been reported.Previous studies showed that the transformants blossom early compared with the wild type and shows early maturity by overexpression of miRNA169d in rapeseed, which is in consistent with the results of previous studies in Arabidopsis.The project intends to identify target genes of iR169d on the basis of previous work,analysis gene expression patterns in the plant and clone target genes of miR169d from Brassica napus,and then transform the target gene over expression vector and RNAi vector constructed to Brassica napus The difference between transformants and wild-type plants will be studied from comparing phenotype,molecular,cellular and physiological levels, and the function of Bna-miR169d and its target gene will be analysised for clarifying the molecular mechanism of miR169d regulation in early development,providing the basis of gene resources and early maturing rapeseed germplasm.