胃癌血清标志物的筛选及检测技术的研究

目的：利用琼脂磁珠核酸信标配基-PCR方法和靶标替换消减SELEX技术从胃癌血清中筛选肿瘤标志物配基，并建立胃癌血清微量蛋白组检测技术。研究内容：用羧基琼脂磁珠作为载体，结合血清标志蛋白，再利用靶标替换消减SELEX技术筛选胃癌血清特异性核酸配基；利用琼脂磁珠分离，及核酸信标配基实时定量PCR 配基传感检测技术（2003年国家自然科学基金资助项目），针对胃癌血清肿瘤标志物，建立血清多标志物分子检测分析体系，为胃癌血清分子检测提供新方法；再反向利用适配体提取特异性标志蛋白并进行蛋白分子鉴定。结果：通过对血清多标志物分子定量系统检测，实现对胃癌的检测监控。

Objective: screening tumor markers from gastric carcinoma serum through agar beads SELEX technology, and establishing a gastric carcinoma serum trace protein detection technology using agar bead nucleic acid beacon aptamer-PCR. Contents: agar beads with a carboxyl group as a vehicle, combined with serum marker proteins, screening specific aptamers from gastric carcinoma serum using target replacement and subtractive SELEX technology; establishing multi-serum molecular markers detection and analysis system using agar beads electrophoretic separation technology, combined with nucleic acid beacon aptamer- real-time quantitative PCR aptamer sensing detection technology(supported by the National Natural Science Foundation in 2003 ); then extracting specific marker proteins reversely using aptamers and identifying the specific marker proteins. Conclusion: Through the quantitative detection of multi-serum molecular markers, improve the detection sensitivity of markers and analysis of dosage – effect relationship, achieve the serum detection and monitoring of gastric cancer.