课题基金基金详情
小分子化合物组合诱导SCAP血管内皮细胞向转分化的实验研究
结题报告
批准号:
81970934
项目类别:
面上项目
资助金额:
55.0 万元
负责人:
张成飞
学科分类:
牙体牙髓及根尖周组织疾病
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
张成飞
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中文摘要
根尖牙乳头干细胞 (Stem cells from apical papilla, SCAP) 具有多向分化潜能,极有可能作为成骨、成血管和成神经等的种子细胞。研究如何促进SCAP向血管内皮细胞高效、稳定分化,获得大量的可临床应用的内皮细胞,能为牙髓血管再生等组织工程和其他缺血性疾病的治疗提供关键的细胞来源。小分子化合物介导的转分化(化学重编程)是一种有广阔前景的、可以改变体细胞谱系命运的方法。已有研究表明,使用小分子化合物组合及特定培养条件,体细胞可被诱导成为各种谱系的目的细胞,如神经前体细胞,神经元细胞、心肌细胞等。我们的研究亦表明,牙源性干细胞具有一定的内皮细胞向分化潜能,但其诱导分化效率较低。本课题拟在前期研究的基础上,优化小分子化合物组合,结合血管内皮生长因子和内皮细胞的特异培养环境,以期高效获得SCAP来源的血管内皮细胞,并进一步研究其细胞特性、体内外功能以及该过程的分子机制。
英文摘要
Stem cells from apical papilla (SCAP) are potentially promising cell sources for tissue engineering due to the isolation with minimal invasiveness and immunocompatibility with autologous origin. SCAP exhibits high proliferation and multipotency. SCAP could differentiate into odontogenic/osteogenic, endothelial and neural-like cells. Endothelial cells (ECs) are the principal building blocks forming the inner layer of blood vessels. However, to generate sufficient numbers of transplantable ECs for translational applications is extremely challenging. Our previous studies demonstrated that dental stem cells showed some extent of potential to differentiate into ECs, but the efficiency of the induction need to be improved. Recently, a new approach has been developed for direct reprogramming one cell type into another targeted cell type with chemical cocktail (chemical reprogramming). Compared with the transgenic approach to induce lineage reprogramming, the chemical reprogramming approach provides a more promising methodology for driving cell lineage switch, avoiding the risk of lentivirus involvement and genome integration through the use of exogenous transcriptional factors. Small molecule compounds are cell permeable, easily synthesized and preserved, and can be precisely controlled in terms of concentration, duration, and combination. Human fibroblasts have been chemically reprogrammed into neural progenitor cells, neuron cells and cardiomyocyte-like cells by different small molecule combinations. It is rational to hypothesize that dental stem cells could be chemical reprogrammed into ECs. The objectives of the present proposal are to induce SCAP to ECs with a set of chemical cocktail we developed, and provide a safe and functional approach to get the candidate cell source for therapeutic angiogenesis.
期刊论文列表
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科研奖励列表
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专利列表
Conversion of stem cells from apical papilla into endothelial cells by small molecules and growth factors.
通过小分子和生长因子将根尖乳头干细胞转化为内皮细胞
DOI:10.1186/s13287-021-02350-5
发表时间:2021-05-03
期刊:Stem cell research & therapy
影响因子:7.5
作者:Yi B;Ding T;Jiang S;Gong T;Chopra H;Sha O;Dissanayaka WL;Ge S;Zhang C
通讯作者:Zhang C
Ang1/Tie2/VE-Cadherin Signaling Regulates DPSCs in Vascular Maturation
Ang1/Tie2/VE-钙粘蛋白信号传导调节血管成熟中的 DPSC
DOI:10.1177/00220345231210227
发表时间:2023
期刊:Journal of Dental Research
影响因子:7.6
作者:Y. Zhang;S. Lin;J. Liu;Q. Chen;J. Kang;J. Zhong;M. Hu;M. Basabrain;Y. Liang;C. Yuan;C. Zhang
通讯作者:C. Zhang
DOI:10.1016/j.joen.2023.10.006
发表时间:2023-12-12
期刊:JOURNAL OF ENDODONTICS
影响因子:4.2
作者:Basabrain,Mohammed S.;Zhong,Jialin;Zhang,Chengfei
通讯作者:Zhang,Chengfei
DOI:10.1016/j.joen.2020.06.024
发表时间:2020-09
期刊:Journal of endodontics
影响因子:4.2
作者:B. Yi;Waruna Lakmal Dissanayaka;Chengfei Zhang
通讯作者:B. Yi;Waruna Lakmal Dissanayaka;Chengfei Zhang
DOI:10.1111/iej.13943
发表时间:2023
期刊:International Endodontic Journal
影响因子:--
作者:Yuchen Zhang;Jialin Zhong;Shulan Lin;Mingxin Hu;Junqing Liu;Jun Kang;Yubingqing Qi;Mohammed S. Basabrain;Ting Zou;Chengfei Zhang
通讯作者:Chengfei Zhang
Ang1/Tie2和VEGF/VEGFR2协同调控牙髓干细胞促进血管稳定的实验研究
  • 批准号:
    --
  • 项目类别:
    面上项目
  • 资助金额:
    55万元
  • 批准年份:
    2021
  • 负责人:
    张成飞
  • 依托单位:
EphrinB2和VEGF协同调控牙髓干细胞促进血管新生的实验研究
  • 批准号:
    81470735
  • 项目类别:
    面上项目
  • 资助金额:
    73.0万元
  • 批准年份:
    2014
  • 负责人:
    张成飞
  • 依托单位:
Bcl-2和cMyc基因调控牙髓干细胞与骨髓来源细胞竞争及促进牙髓再生的实验研究
  • 批准号:
    81271135
  • 项目类别:
    面上项目
  • 资助金额:
    70.0万元
  • 批准年份:
    2012
  • 负责人:
    张成飞
  • 依托单位:
国内基金
海外基金