PAHs对抗生素抗性质粒的物理性损伤及机制

多环芳烃（PAHs）与抗生素抗性质粒（Arps）常共存于环境中，危害人群健康和生态安全，PAHs-Arps复合污染的风险已引起学界关注。弄清PAHs与Arps间的交互作用是阐释其复合污染的前提。已证实，PAHs能影响普通DNA内氢键、碱基对芳香环堆积力、碱基间范德华力，使其发生物理性损伤。然而迄今，关于复合污染背景下PAHs对Arps的物理损伤，相关研究鲜有报道。本项目以常见PAHs和Arps为目标污染物，拟采用微滴定、基因操作等试验方法，利用谱学、计算化学、原子力学等分析技术，在弄清两者互作方式的基础上，系统地揭示PAHs对Arps的物理性损伤规律；研究Arps内抗性基因的转录与表达，找出其损伤程度与基因表达水平间内在联系，评估其损伤的生物学效应，综合性地阐释Arps损伤的机制。成果可为深入揭示PAHs-Arps复合污染原理提供理论基础，对防治其复合污染、保障生态安全具有重要意义。

Polycyclic aromatic hydrocarbons (PAHs) and antibiotic resistance plasmids (Arps) in environments pose a serious threat to human health and ecological security. PAHs-Arps combined pollution has aroused much public concern. It is necessary to understand the fundamental rules for PAHs-Arps interaction prior to assessing the underlying environmental ecological risks for PAHs-Arps combined pollution. Previous reports have shown that PAHs have an influence on hydrogen bonds, hydrophobic effect between bases and aromatic rings, and Van der Wals forces between bases, physically damaging the DNA structure. But little is known about the physical damage of Arps induced by PAHs in the background of PAHs-Arps combined pollution. In the present study, PAHs and Arps species frequently detected in environments are selected as target pollutants. Research methods including the micro titration, and genetic manipulation etc. and analysis technologies including spectroscopy, computational chemistry, and atomic force microscope are employed in the study to: (1) systematically reveal the fundamental rules of PAHs-induced physical damage to Arps based on the interaction between PAHs and Arps; (2) study the transcription and expression of resistance gene in Arps, identify the relationship between the extent of damage and gene expression level, evaluate the biological effects of damage, and comprehensively expound the mechanisms of PAH-induced physical damages to Arps. It is expected that the information obtained from the study may provide a theoretical foundation for revealing the principle of PAHs-Arps combined pollution, which has great significance for controlling the combined pollution and protecting ecological security in region.