KPS调控鸡免疫缺陷活性成分的结构解析及分子机制

针对家禽免疫抑制性疾病病原可引起免疫细胞减少、功能降低，导致神经-内分泌-免疫网络紊乱的现状，提出大叶南五味子多糖可以直接作用于免疫效应细胞(细胞毒性T 细胞、B 细胞等)、抗原递呈细胞（单核细胞、巨噬细胞等）上的TLR受体，或经cAMP-PKA路径促进NF-κB核转录因子的分泌而实现免疫调节的假说，用MTT法检测大叶南五味子多糖对雏鸡T淋巴细胞、B淋巴细胞、腹腔巨噬细胞增殖能力的影响，确证其作用的靶细胞；用15日龄雏鸡制备免疫抑制病理模型，应用ELISA方法检测多糖对免疫抑制雏鸡外周血中重要细胞因子如IL-1、IL-2、TNF、IFN等的含量和cAMP含量的影响；用比色分析法检测NF-κB等核转录因子的含量；用GC、MS和NMR等方法对调节免疫缺陷活性成分进行结构解析。以此阐明大叶南五味子多糖的免疫调节作用机制及其物质基础，为其应用于家禽免疫抑制性疾病的预防和治疗奠定基础。

For poultry immunosuppression disease pathogeny can cause immune cytoreduction, and function decrease, neural-endocrine-immune network disordered. The hypothesis of immune regulation mechanism was suggested that kadsura marmorata polysaccharides(KPS) could promote the NF-kappa B nuclear transcription factors(NF-κB) to secrete by directing toward Toll-like receptor(TLR) on the surface of immune effective cells (such as T lymphocyte,B lymphocyte,etc) and antigen presenting cells (monocytes,macrophages,etc),or the cAMP-PKA path. The target cell of KPS was confirmed by the immune cell reproductive test. This test was detected cell reproductive activity of T lymphocyte, B lymphocyte and peritoneal macrophage from chicken by MTT method.Immunosuppression pathology model was made with the fifteen days-aged chicken. The effect of KPS on the contents of important cell factor as interleukin-1(IL-1), interleukin-2(IL-2), alpha tumor necrosis factor(TNF-α) and gamma interferon(IFN-γ) and cAMP in outside blood from immunosuppression chicken's were detected by ELISA method. The content of NF-κB was detected by colorimetric DNA-binding assay. The structure of regulation immune deficiency active polysaccharide was analyzed by gas chromatography(GC), mass-spectrography(MS), nuclear magnetic resonance (NMR) method and so on. The immune regulation mechanisms and material base of KPS were clarified. And this lay the foundation for prevention and treatment of immunosuppression disease in the poultry.