大白菜复等位基因雄性不育资源既是杂种优势利用的优异不育种质，也是研究高等植物雄配子发育的理想突变体资源。在前期研究中，申请者分析了该系统雄性不育两用系"AB02"可育株和不育株的转录组差异，发现钙结合蛋白基因BrCaBP1和BrCaBP2在不育株上受到强烈抑制。本项目拟利用组织原位杂交和GFP融合基因表达研究BrCaBP1和BrCaBP2的时空表达模式及亚细胞定位；利用RNAi技术，分别抑制大白菜和菜心中BrCaBP1和BrCaBP2的转录，鉴定转基因植株后代的育性变化，同时利用第二代测序技术对转基因植株和野生型植株的的花蕾转录组进行深度测序，并同已有的"AB02"系统花蕾转录组测序数据相比较，探讨钙信号传导途径在大白菜复等位核不育系统育性调控中的可能作用机制。本项目成果对深入研究高等植物雄配子发育的内在调控机制具有重要意义，也将为大白菜复等位基因雄性不育资源的深入利用奠定理论基础。

The multiple allele inherited male sterile material in Chinese cabbage is not only an excellent germplasm in heterosis breeding，but also an ideal mutant resource for studing the male gametogenesis development in plants. In previous studies, the applicant analyzed the transcriptome differences between the fertile and sterile plants in the male sterile line "AB02" and found that two calcium-binding protein genes, BrCaBP1 and BrCaBP2, were strongly inhibited in the sterile plants. In this study, the temporal and spatial expression and subcellular localization of BrCaBP and BrCaBP2 will be analyzed by using in situ hybridization and GFP fusion expression.This study is aimed to silence the expression of BrCaBP1 and BrCaBP2 in Chinese cabbage and flowering Chinese Cabbage by RNAi,respectively,and then identify the fertility change in the transgenic progeny.The bud transcriptomes of transgenic progeny and wild type plants will be analyzed by the second generation sequencing techniques, and compared with the bud transcriptomes of "AB02" to discuss the probable mechanism of calcium signaling pathway in the fertility regulation of the multiple allele male sterility of Chinese cabbage.The foudings will be valuable for further understanding of mechanism of the male gametogenesis development in plants，and lay a theoretical foundation for better utilizing the multiple allele male sterile germplasm in Chinese cabbage.