氧化应激过程中产生的活性氧（ROS）会损伤细胞，有可能引起癌症、神经性疾病、帕金森病等生理病变。由于有效直接测定ROS分析方法的匮乏，生物体内氧化应激状态与相关疾病之间关系的研究受到一定制约。本项目立足于电化学技术与生命科学的交叉研究，以活性氧及氧化应激过程密切相关的生物分子为研究对象，以扫描电化学显微镜(SECM)为手段，致力于氧化应激过程中活性氧与DNA 等生物分子的相互作用机理研究；建立灵敏度高、响应速度快的活性氧检测新方法；建立氧化应激损伤的评价体系。在此基础上，对细胞中的活性氧释放规律进行实时在线检测，探索氧化应激、活性氧物种与引起相关疾病之间的相关性。该项目的完成将为生物体中活性氧的直接检测提供技术平台，为研究活性氧浓度的变化在细胞生理活动中的作用提供重要的实验手段，为疾病的诊断，抗癌抗衰老药物的筛选提供实验基础。

Reactive oxygen species (ROS) including superoxide anion, hydrogen peroxide, and hydroxyl radical, are excess generated in vivo during oxidative stress processes. The overproduction of ROS may damage the cells and cause cancer, Neurological diseases and Alzheimer’s disease. Due to the lack of effective analysis method for the direct determination of ROS, it is subject to certain restrictions to study the relationship between oxidative stress in living organismsis and related diseases. This program is about the intersisciplinary study between electrochemistry and life science. It focuses on the research of interaction between reactive oxygen species and biological moelcules (include DNA) in oxidative stress processes by means of scanning electrochemical microscopy (SECM). We will establish new methods to detect ROS with high sensitivity and fast response, as well as the evaluation system of oxidative stress damage. Based on this, we will carry out the real-time online detection for ROS that release by the cell itself, and explore the relationshp among the ROS species, oxidative stress and the related disease. After finishing this program, it will provide a platform for the direct determination of ROS in living organismsis, and an important experimental method for investigation the effect of the ROS concentration changes on the cell physiological activities. Furthermore, it will provide the experiment base for the diagnosis of the disease, screening of anticancer and anti-aging drugs.