结核病是单因素危害人类健康最严重的疾病。耐药菌株的出现和艾滋病合并感染使结核成为我们面临的最大公共卫生挑战。通过"结核分枝杆菌压电测试新技术研究和应用"课题的研究，发现以培养法为基础建立的分析检测方法，检测速度受结核分枝杆菌分裂周期长限制，无法再提高。本课题拟在前课题的基础上，构建基于适配体分子识别的结核分支杆菌电化学传感测试新技术。利用适配体对靶细胞的特异性识别和电化学传感器对电参数的灵敏响应，实现结核分枝杆菌的快速特异检测。所构建的传感方法既能克服培养法受细菌增代时间限制的缺点，又能克服抗原抗体法单克隆抗体难得，假阳性太高的缺点；具有简单、快速和特异性，满足临床检测需求。 具体内容有：⑴筛选用于检测的结核分枝杆菌适配体；⑵制备基于核酸适配体的电化学敏感探针；⑶建立基于核酸适配体响应的结核分枝杆菌电化学检测新方法⑷ 分析统计评价所建分析方法在临床应用的结果。

M.tuberculosis is the most serious disease of single factor hazard to human health. The appearance of resistance strains and AIDS co-infection have made the TB become the biggest challenge of public health we have to face.Through the research process of " the researching and application of new M.tuberculosis detection technology based on piezoelectric sensor", We have found that the detection time of M. tuberculosis based on the cultivation method were too long to meet the demands of TB control and prevention because of its long generation cycle.Based on the research results of previous project, we plan to construct new M.tuberculosis detection technologies based on the sensitive response of electrochemical sensor to electric parameters and specific recognition of aptamer.M.tuberculosis will be rapid and specific detected by this new method.The constructed method will meet the demand of clinic test with characteristics of simple, rapid and specific, not affected by the cycle time of T. tuberculosis.It will overcome the disadvantages of method based on antigen-antibody reaciton, such as high false positive results and difficult to obtain monocolone antibody.The main contents are described as follows: ⑴ the aptamer screening for the detection of M. tuberculosis by the TECS-SELEX methods; ⑵ preparation of electrochemical probe based on aptamer; ⑶the estalishment of rapid detection of M. tuberculosis based on aptamer response;⑷Application and evaluation of new detection methods in the assay of actual sample analysis by statistical method.