土壤有机磷是生物可利用磷的重要来源，生物炭在促进有机磷矿化利用方面具有巨大的应用潜力，但其对土壤有机磷矿化过程的影响及潜在作用机理尚不明确。本研究以不同pH值的红壤、棕壤和潮土为供试土壤，以水稻、小麦和玉米秸秆制备供试生物炭，以玉米为供试作物，利用靶位宏基因组测序技术研究生物炭添加对不同土壤微生物来源磷酸酶基因群落特征的影响及其与磷酸酶活性的关系；利用液体31P核磁共振技术与分光光度法等研究生物炭在不同土壤施用后对有机磷组分及各自专性磷酸酶活性的影响，以探明生物炭添加对土壤有机磷酶促矿化过程的影响及其潜在作用机理；并在此基础上研究生物炭添加条件下土壤有机磷矿化与作物磷吸收之间的关系，为利用生物炭调控土壤有机磷转化及促进作物磷吸收、推动生物炭在化肥减施增效中的应用提供理论依据。

Organic phosphorus (P) is a substantial component of the soil bioavailable P, there are increasing evidence suggested that biochar application enhance organic P mineralization, but the underlying mechanisms are still not clear. In present study, red, brown and calcareous Fluvo-Aquic soils with different pH were treated with three kinds of biochar (rice, wheat and corn straw) application, microbial gene clusters encoding phosphatase, phosphatase activities and organic P fractions are investigated by gene-targeted metagenomics approach, spectrophotometric method and 31-P nuclear magnetic resonance (NMR) to explore their response to biochar additions. Relationships between gene clusters encoding phosphatase, phosphatase activities and organic P fractions in soil are analyzed by structural equation modeling (SEM) and redundancy analysis (RDA) to investigate the microbiological and molecular biological mechanisms involved in organic P mineralization under biochar additions. This study will improve our understanding of the regulatory mechanisms of biochar application in soil organic P mineralization and crop P acquisition, and provide theoretical foundations for promoting biochar application and reducing chemical fertilizer inputs in agroecosystems.