自然药学观是指导中药理想药物研究的重要学说，核心是关联分析发现最佳药物体系的中药-成分-效应表征库。体外药理研究在药物活性筛选、细胞分子药理研究起重要作用，但血清药理因内源性成分干扰而重现性差，且体内药代特征而成分含量低，难以准确地把握三者间关系；含药肠吸收液无代谢过程故缺乏活性代谢物。基于上述分析，我们提出“含药肝孵育液”的新方法：制备经离体肠吸收并肝细胞孵育的含药体系用于体外药理研究，可解决上述问题并将PK-PD直接呼应起来。本研究以麻杏石甘汤混料均匀设计配方为对象，采用含药肝孵育液法、Pearson分析，多水平构建疗喘的效应矩阵；联合URLC-QTOF/MS检测、偏最小二乘回归分析，构建疗喘的成分矩阵；经关联分析两矩阵间关系、发现各局域网络体系表征库，初步揭示较优药物体系的配伍规律。通过研究探寻更适合中药体外药理研究的方法，以深化认识中药药物体系的本质及内涵，为理想药物研究提供示范。

The Natural Pharmacy is an important guiding ideology for the research of ideal drug of TCM, core of the theory is to association analysis and find network system’s characteristics library of optimal drug system about TCM-Components-effects. In vitro pharmacological plays an important role in drug screening, study on cellular and molecular pharmacology. However, the serum pharmacological/pharmaceutical chemistry was poor reproducibility due to the interference of endogenous components, and the in vivo pharmacokinetic characteristics caused low content components, thus it is difficult to accurately grasp the relationship between the three. Although the intestinal absorption liquid containing medicine composition can solve the problem of serum, but lack of metabolic process. Based on the above considerations, we further propose a new method, the method of liver incubated buffer containing medicine. That is prepare liquid containing medicine composition in vitro pharmacokinetic process and use it at in vitro pharmacological study, the method can directly echo PK-PD process. In this study, formula of Maxingshigan Decoction which’s designed by mixture homogeneous design was studied as the object, the method of liver incubated buffer containing medicine, technology of HCS and RT-PCR, Pearson analysis were used to construct antiasthmatic effective matrix from multi-level. Technology of URLC-QTOF/MS and partial least squares were used to construct component matrix of antiasthmatic. Principal component analysis and correlation analysis were used to find each layer local area network system, thus, to reveal the essence and law of the compatibility of the optimal drug system. Through the study, we hope to explore a new method in vitro pharmacological research more suitable for TCM, deepen the understanding of the essence and connotation of the TCM drug system, and provide demonstration for the research of the ideal drug.