基于代谢组学的溃疡性结肠炎大鼠肠道微生态变化及安肠汤干预机制研究

溃疡性结肠炎(UC)是一种慢性炎症性肠病，由于该病发病率逐年增加，发病机制尚未完全阐明，又缺乏有效的治疗药物，故探究其发病机制及寻找有效防治药物一直是研究热点。肠道微生态失调在UC的发生发展中具有密切关联性，但两者之间的因果关系及关键因素靶点仍不明确，而研究方法的不足和缺陷限制了更深入的研究。代谢组学作为一门新兴学科，是通过检测机体在生理病理活动过程中的代谢产物，来推究机体生命活动发生变化的机制。而采用代谢组学来探索UC的肠道微生态变化，目前国内外尚未见有相关报道。安肠汤是课题组临床应用和研究20余年治疗UC的中药复方，临床疗效佳，无明显毒副作用。本课题通过建立 UC 大鼠模型，运用代谢组学、分子生物学分析UC大鼠代谢组学特征及肠道微生态变化特征，探索UC发病机制，观察安肠汤对UC大鼠肠道微生态的干预作用，揭示其防治UC的作用靶点。

Ulcerative colitis (UC) is a chronic inflammatory bowel disease, and it has been researchful hotspot that to explore its pathogenesis and look for effective drugs, due to increasing incidence year by year, undefined pathogenesis and lack of effective drug treatment to UC.There is close relationship between Intestinal microflora disorders and UC during its occurrence and development,but it was still unclear that causality and key factor's target point between the two. However, further deep research is restricted by shortcomings and defects of methods. As a new branch of science, metabolomics is through detecting metabolites in the body during the process of physiology and pathology,thus to explore the mechanism of the change about activities of body.But it has not been seen relevant reports at home and abroad about exploring the intestinal microecological change of UC on adopting metabonomics. Anchang decoction is compound Chinese herbs that we have applicated and researched in clinic more than 20 years，its clinical effective is well and no obvious toxic side effect. This study would get to analysis metabonomics'characteristics and change characteristics of intestinal microflora of UC rats by establishing the UC model of rats,putting to use metabonomics and molecular biology,in order to explore the pathogenesis of UC and observe the change in intestinal microflora of UC rats intervened Anchang soup decoction,and accordingly reveal the target spot to prevent UC by anchang decoction.