本课题将以荧光金属纳米团簇和碳点为基础，构建一系列新型的碳点-贵金属纳米团簇比率荧光复合材料，并将其研制成生物传感器，应用于几种与疾病相关的生物酶标志物的定量检测中。首先，荧光金属纳米团簇具有比表面积大、生物相容性好等优势，与稳定性好、无毒且荧光性能良好的碳点通过硅烷化试剂以共价键或静电作用形成发射双波长的比率荧光纳米粒子；对该比率荧光纳米粒子的形貌结构、尺寸、光学性质进行表征；进而对材料的特异性、灵敏度进行检验，并探讨其与分析物的荧光相互作用机理；最终实现在复杂样品中对生物酶标志物的高灵敏、高选择性检测，并进一步应用于细胞生物成像研究。

This work will focus on manufacturing and applying combined fluorescent metal nanoclusters and carbon dots. First, this study initiates to synthesize a series of novel ratiometric fluorescent material based on conjugating fluorescent metal nanoclusters and carbon dots. Furthermore, we will develop multiple biosensors for the detection of several enzyme biomarkers associated with cancers. The fluorescent metal nanoclusters possess high specific surface areas and good biocompatibility, while carbon dots exhibit superior stability, nontoxic, and fluorescent properties. Based on this, a series of dual emissive ratiometric fluorescent nanocomposites would be fabricated using step-by-step assembly strategy by covalent binding or electrostatic interaction. The structures, size distribution, and spectral characteristics of these ratiometric fluorescent nanocomposites were systematically optimized. Furthermore, combining the surface functionalization and design principle of optical sensors with the properties of fluorescent nanomaterials, we aim to develop high sensitive and selective sensing platforms for the detection of enzyme markers in real biological samples, facilitating their applications in cell fluorescence imaging.