前期研究发现藏红花素与顺铂联用增加抑制肿瘤增殖作用。我们利用全基因表达谱芯片等表达策略检测到前列腺相关基因（PAGE4）在藏红花素联合顺铂处理的胃癌细胞表达下调，在胃癌组织中呈现高表达。提示PAGE4是联合化疗潜在的重要靶分子。PAGE4 是癌-睾丸抗原家族成员之一，但未有其参与胃癌发生发展和化疗敏感性调控的报道。本课题将利用重组慢病毒介导的PAGE4干涉策略，在细胞模型验证PAGE4调控胃癌细胞增殖、转移、凋亡等生物学特性的作用和机制；探讨PAGE4在胃癌细胞藏红花素联合顺铂敏感性中作用及相关性；利用胃癌荷瘤模型，验证过表达 PAGE4对藏红花素联合顺铂抑制胃癌生长的作用；结合临床胃癌组织标本及病例分析，确定PAGE4与胃癌发生和发展的临床相关性。该课题将揭示PAGE4调控胃癌发生和发展的分子新机制，为临床治疗胃癌提供潜在治疗靶点，并为藏红花素和顺铂联用提供理论基础。

Base on our previous work, we identified that the sensitivity of cisplatin on gastric cancer were enhanced by combined with crocin. By using the gene chip and other expression strategies we found that proto oncogene prostate related gene 4 (PAGE4) was significantly decreased by treating with crocin and cisplatin. Further we found that PAGE4 was over expressed in gastric carcinoma tissue. It indicated that PAGE4 is a potential target molecule for combined chemotherapy. PAGE4 is a member of the cancer-testicular antigen family, whether it involved in the development of gastric cancer and the regulation of chemotherapy sensitivity was ambiguous. In this study, qPCR was used to identify the recombinant lentivirus-mediated PAGE4 interference strategy, MTS assay, flow cytometry, qPCR and western blot was used to verify the role and mechanism of PAGE4 in regulating the proliferation, metastasis and apoptosis of gastric cancer and the sensitivity of the combination of crocin and cisplatin both in vitro and in vivo. PAGE4 overexpression tumor bearing mice model was used to investigate the effect of PAGE4 on the gastric cancer growth inhibition of treated with the combination of crocin and cisplatin. To use the clinical gastric cancer specimens and case analysis were utilized to determine the clinical relevance of PAGE4 and the development of gastric cancer. This topic will reveal the new mechanism of PAGE4 on the gastric cancer development and progression regulation, and provide a potential therapeutic target for the clinical treatment of gastric cancer, and offer a theoretical basis for the combination of crocin and cisplatin.