基于多组学分析技术揭示残翅病毒（DWV）干扰蜜蜂翅型分化和发育的机制研究

残翅病毒（DWV）作为蜜蜂上流行率最高的病毒，其与狄斯瓦螨的协同作用被认为是蜂群健康的主要威胁因子之一。DWV不仅造成蜜蜂寿命缩短，最主要的影响是导致蜜蜂翅型分化和发育异常而出现残翅等症状，但至今人们对造成DWV由隐性感染向显性感染转变的机制仍知之甚少。本项目基于前期的研究基础和DWV组织特异性检测及分型鉴定的结果，拟借助实验室已经建立的人工转染蜂螨、病毒接种以及添加饲喂杀虫剂等方法构建残翅蜜蜂模型，通过基因组、转录组和蛋白质组等多组学分析技术挖掘DWV干扰蜜蜂翅型分化和发育的关键基因，并重点关注DNA甲基化和微小RNA在蜜蜂表观遗传修饰中可能起到的作用。随后，利用荧光定量PCR、人工饲养蜜蜂幼虫及RNA干扰等技术进行结果验证，以此探究DWV干扰蜜蜂翅型分化和发育的致病机理，为蜂群内DWV和狄斯瓦螨的有效防控提供新的思路和策略，促进养蜂业的健康发展。

As the most popular virus on honeybees, the interactions between deformed wing virus (DWV) and Varroa destructor are considered as one of the main threats to honeybee health. Not only does DWV shorten the longevity of honeybees, but also it causes the main symptom of deformed wings due to the abnormal differentiation and development of the wings. However, little is known about the mechanism that causes the transformation of DWV from covert to overt infection. Based on the previous researches and the results of tissue specificity detection and variants identification of DWV, the project intends to construct the deform-winged honeybee models by the laboratory-established means of artificial infestation of mites, virus inoculation as well as insecticide feeding, and to explore the key genes involving the wing differentiation and development in honeybees that perturbed by DWV via multi-omincs analysis such as genome, transcriptome and proteome. The DNA methylation and micro RNAs will be paid close attention to their potential roles in mediating the epigenetic modification of honeybees. Subsequently, fluorescent quantitative PCR, artificial rearing of bee larvae and RNA interference were used for the result verification, so as to explore the pathogenic mechanism of DWV-perturbed wing differentiation and development in honeybees, provide new ideas and strategies for effective prevention and control of DWV and V. destructor in honeybee colonies, and promote the healthy development of beekeeping.