器官的缺损和功能衰竭是人类健康所面临的主要问题之一，也是许多疾病致死的重要原因。促进损伤器官的修复与再生是恢复器官功能、提高人类生存质量的重要途径。果蝇的翅成虫盘具有强大的再生能力，是研究器官再生机制的重要模型。细胞重生是近几年新发现的细胞在凋亡压力下激活了效应caspase分子后存活的过程。细胞重生可以用来减少组织在短时致死压力下的损伤,以维持组织和器官的功能。我们的前期工作建立了在果蝇体内追踪细胞重生的报告体系，并利用该体系发现果蝇的翅成虫盘在辐射损伤后有大量激活了效应caspase分子的细胞通过细胞重生存活，并且再生的翅成虫盘主要由重生的细胞分裂增殖形成，而非之前认为的由未启动凋亡程序的细胞增殖而成。本课题将在此基础上研究细胞重生在器官再生中的必要性、细胞重生后的行为以及决定细胞重生能力的分子机制。这一研究可以拓宽我们对组织和器官再生过程的认识，并为提高人类器官再生能力提供新的靶点。

Organ loss and failure is one of the main threats of human health and a major cause of death in many diseases. Promoting the repair and regeneration of damaged organs is an important strategy to restore organ function and improve human life quality. Drosophila wing imaginal disc is a commonly-used model to study the mechanism of regeneration due to its strong regenerative capacity. Anastasis is a process by which cells survive executioner caspase activation following transient exposure to a lethal dose of apoptotic stimuli. Anastasis can be used to limit the permanent damage following transient lethal stress, thus to maintain organ function. Previously, we have established a lineage tracing system in Drosophila to mark all cells that have undergone anastasis and their progenies. Using this system, we found many cells in Drosophila wing imaginal disc can survive executioner caspase activation after X-ray radiation and the regenerated disc is formed through proliferation of the anastatic cells, different from the traditional view that regeneration is conducted by undamaged cells without apoptosis initiation. Based on these data, we will study the necessity of anastasis in organ regeneration, the cell behaviors after radiation-induced anastasis, and the determinants of the capacity of cells to undergo anastasis in response to lethal stress. This study will broaden our understanding of the mechanism of organ regeneration and provide new targets for improving the regenerative capacity of human organs.