倭蛙是青藏高原的特有物种，而且是此高原除东南部外的广大地区内少有的几种无尾两栖类动物之一，在当前全球变暖和两栖动物种类和数量大幅减少的背景下，该物种的前途如何是一个非常值得关注的问题。要想预测该物种的前途，一是要进行野外种群动态监测，另一个方法则是开展生理生态研究，探究温度变化对该物种生理活动的影响。本项目拟采用经典的实验方法，研究温度对倭蛙蝌蚪、幼蛙和成体生理功能的影响，具体内容包括温度对倭蛙胚胎发育的影响、对蝌蚪运动能力和代谢率影响、温度对成体倭蛙运动能力、心率和静止代谢率的影响等，同时本项目还拟通过对转录组和代谢组的分析，探讨温度对倭基因表达和代谢路径的影响，探究宏观指标的变化与基因表达和代谢途径之间的关系。通过本项目的研究，预期可以获得温度变化对倭蛙发育不同阶段生理机能的影响模式，并探讨其内在机理，分析预测在全球变暖背景下倭蛙可能遭遇的挑战和物种的前途，为倭蛙的保护提供参考。

The frog Nanorana pleskei is an endermic species to Qinghai-Tibet Plateau and one of the few species of anura distributing on the most area of the plateau except the southeast part. Based on the fact of global warming and that the species number and population number of amphibians in the world decline tremendously the future of this species is a very important issue to pay close attention. To predict the fate of this species on one hand we should monitor its population dynamics, and on the other hand we can study the eco-physiological properties. That is to study the effect of temperature variation on the physiological activities. In this project we plan to test effects of temperature on the physiological functions of the tadpoles, juveniles and adults of Nanorana pleskei. The functions to be tested include embryonic development, the locomotion ability and metabolic rate of tadpoles and adults, the heart rate of adults. Meanwhile we also plan to study the effect of temperature on the transcriptome and metabolome of this frog so that we can analyze the relation between the physiological function and the gene expression as well as the metabolic pathways. Through the research practice of this project we expect to know the patterns that temperature affects the physiological functions of this frog and to discuss the mechanism of these effects. We can also analyze and predict the future the challenges of Nanorana pleskei on the background of global warming and provide the references in the conservation of this species.