细胞表面糖基与细胞生理活动息息相关，其异常表达与细胞增殖、肿瘤发生发展、肿瘤恶化和转移等有着密切联系。然而，细胞表面糖基种类众多，其表达变化异常复杂，传统体外检测方法难以在单细胞层面对其组成和分布进行准确的分析与定位。因此，本项目拟发展一种基于飞升级超微电渗流的电化学调控技术，结合超灵敏微操作平台与多通道显微镜，利用尖端直径小于100纳米的超微玻璃通道精确靶向单个细胞特定位点，通过超微电渗流控制外源性糖类或人工纳米药物的注入剂量，针对不同糖基分子及相关通路进行修饰或调控，最终实现对单细胞表面糖基表达与分布进行精准、快速的可视化分析，并对细胞糖基化通路进行人工干预，从单细胞水平上深入揭示细胞表面糖基异常与肿瘤等恶性疾病的联系。本研究体现了化学、生物学与医学等多学科的交叉，有望通过超灵敏电化学方法为单细胞表面糖基的高精准分析与可控编辑提供新的技术平台，进而推动生命科学和医学的发展。

Cell surface glycans are closely related to the physiological activity of the cells, and the abnormal expression of these glycans is relevant to cell proliferation, tumorigenesis, progression and metastasis. However, there are a wide variety of glycans on cell surface with extremely complex changes in expression. In this case, the traditional in vitro assays cannot perform the precise analysis of the glycan composition and expression at single cell level. Therefore, in this project, we plan to develop an electrochemical control technique based on femto-litre grade ultramicro-electroosmotic flow. By combining with an ultrasensitive manipulator and a multichannel microscope, a nanopipette with the tip diameter less than 100 nm is used for the precise pointing of a specific site at single cell level. Via the ultramicro-electroosmotic flow, the injected dose of exogenous glycans or artificial nanodrugs can be controlled for the modification or regulation of various glycan molecules and the related glycosylation pathways. Ultimately, we hope to realize the precise and fast visualization analysis of the glycans on single cell surface, and to perform manual intervention to cellular glycosylation pathways, in order to reveal the internal connection between the abnormal cell surface glycan expression and malignant diseases such as cancer. The study reflects the integration of chemistry, biology, medical science and etc. It is hoped that the ultrasensitive electrochemical method will offer a novel technical platform for the precise analysis and controllable editing of glycan on single cell surface, thus promoting further development of life science and medical science.