维持软骨稳态是解决软骨退变问题的关键点之一。在前两个国自然的资助下，证实了软骨细胞外基质合成分解代谢与关节软骨退变密切相关，同时，ncRNAs在表观遗传、转录和转录后水平的调控在成软骨分化及软骨退变过程中起到重要作用；在这基础上，我们对可能起关键作用的ncRNAs做了进一步探讨，通过芯片测序，发现超级增强子lncRNA与软骨退变密切相关，我们对此现象进行了预实验，结果证实se-lncRNA RP13-516M14.1可能通过SOX9和miR-490/Smad3调控轴上调软骨特异性基因。我们推断lncRNA RP13-516M14.1可能在维持关节软骨稳态方面起到正向调控作用，因此设计本研究，旨能通过体内外实验阐明以超级增强子lncRNA RP13-516M14.1为核心的调控网络在软骨分化发育过程中的作用机制，并利用该机制维持软骨稳态、延缓软骨退变。本研究是以往研究基础上的进一步探讨。

Maintaining cartilage homeostasis is one of the key points to solve the problem of cartilage degeneration. With the support of the former two NSFCs, it has been confirmed that the anabolism of cartilage extracellular matrix is closely related to the degeneration of articular cartilage. Meanwhile, ncRNAs play an important role in the regulation of epigenetic, transcriptional and post-transcriptional levels in the process of chondrogenic differentiation and cartilage degeneration. Based on this theory, we took a further step to explore whether ncRNAs play key roles in this process. After analyzing of the result of the se-lncRNA chip sequencing that we have done, we found the super enhancer lncRNA RP13-516 M14. 1 does have close correlation with cartilage degeneration. We conducted preliminary experiments on this phenomenon, and the results confirmed that se-lncRNA RP13-516M14.1 may up-regulate cartilage specific genes through SOX9 and mir-490/Smad3 axis. We infer that lncRNA RP13-516M14.1 possibly play positive roles in maintaining the homeostasis of articular cartilage. Therefore, this study was designed to confirm the mechanism of the regulatory network with super enhancer lncRNA RP13-516M14.1 in the process of cartilage differentiation and development through experiments in vivo and in vitro, and to utilize this mechanism to maintain cartilage homeostasis and delay cartilage degeneration. This study is a further study on the basis of previous studies.